Product nameAnti-TEX12 antibody
DescriptionRabbit polyclonal to TEX12
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- Human testis tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Tissue specificityTestis specific.
- Information by UniProt
- Testis expressed gene 12 antibody
- Testis expressed sequence 12 protein variant 3 antibody
- Testis-expressed sequence 12 protein antibody
Immunofluorescent staining of Human cell line U-251 MG shows positivity in centrosome. Recommended concentration of ab122455 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lanes 2-3 : Anti-TEX12 antibody (ab122455)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing TEX12 with a C-terminal myc-DDK tag (~3.1kDa)
ab122455, at 1/200 dilution, staining TEX12 in paraffin embedded Human testis tissue by Immunohistochemistry.
ab122455 has not yet been referenced specifically in any publications.