Product nameAnti-Tex14 antibody
Predicted to work with: Mouse
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
|IHC - Wholemount||1/3000.|
RelevanceTestis-expressed gene 14 (TEX14) is a novel protein that localizes to germ cell intercellular bridges. In the absence of TEX14, intercellular bridges are not observed. Spermatogenesis in Tex14–/– mice progresses through the transit amplification of diploid spermatogonia and the expression of early meiotic markers but halts before the completion of the first meiotic division.
- Cancer/testis antigen 113 antibody
- CT113 antibody
- Inactive serine/threonine protein kinase TEX14 antibody
Ab41733 in IHC on PFA fixed adult mouse testis with microwave antigen retrieval. This antibody gave the expected staining pattern of intercellular connections between germ cells (brown dots). It also gave some background staining of sperm tails (in the centre of the tubules). For more information, please see abreview.
This antibody appears to stain intercellular bridges between germ cells, as it should. TEX14 staining is present within the testis cords between between DDX4 positive cells (germ cells), but is absent between DDX4 negative cells (Sertoli cells).
Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde for 5 hours at 4°C, then washed overnight in PBS at 4°C. Testes were detunicated and cut in half, then permeabilized with 0.1% Triton X-100 in PBS for 30 min at 20°C, then blocked with 3% BSA with 0.1% Triton X-100 in PBS for 1 hour at 20°C. Sections were incubated with Rabbit Anti-Tex14 (Abcam ab41733) diluted 1/3000 plus Goat Anti-DDX4 diluted 1/800 for 14 hours at 4°C. Tissue was then washed 3X for 30 min each with 0.1% Triton X-100 in PBS. Tissue was then incubated with Donkey Anti-Rabbit 555 (Abcam ab150062) plus Donkey Anti-Goat 647 (Abcam ab150155) diluted 1/500 for 3 hours at 20°C. with Abcam DAPI after washing 3X with 0.1% Triton X-100 in PBS. Tissue was then incubated with Fluoroshield Mounting Medium With DAPI (Abcam ab104139) with mounted onto a slide and coverslipped. Tissue was imaged on an Olympus FV 1000 confocal microscope and represented as a maximum intensity Z-projection of a 5 µM thick Z-stack.
ab41733 has been referenced in 3 publications.
- Miyauchi H et al. Bone morphogenetic protein and retinoic acid synergistically specify female germ-cell fate in mice. EMBO J 36:3100-3119 (2017). PubMed: 28928204
- Rabionet M et al. Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors. Hum Mol Genet 24:4792-808 (2015). PubMed: 26045466
- Lorès P et al. Deletion of MgcRacGAP in the male germ cells impairs spermatogenesis and causes male sterility in the mouse. Dev Biol 386:419-27 (2014). Mouse . PubMed: 24355749