Overview

  • Product name

    Anti-TEX15 antibody
  • Description

    Rabbit polyclonal to TEX15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 1473-1591 of Human TEX15

  • Positive control

    • Human testis tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122364 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122364, at 1/500 dilution, staining TEX15 in paraffin-embedded Human testis tissue by immunohistochemistry.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Answer

I have run an alignment for ab122364 against mouse Tex15. Unfortunately the results of this BLAST were very poor and we would not recommend this item for use in mouse samples. Identities = 51/118 (43%), Positives = 77/118 (65%), Gaps = 13/118 (11%)

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