Overview

  • Product name

  • Description

    Rabbit polyclonal to TEX19
  • Host species

    Rabbit
  • Specificity

    Immunogen has 86% homology with mouse TEX19 sequence. However, customer feedback indicates that the results for mouse are non-specific (see Abreviews).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    14 amino acids long synthetic peptide within amino acids 108-157 of Human TEX19

  • Positive control

    • Human placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-TEX19 antibody (ab90953) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 18 kDa



    Gel concentration: 10-20%

References

ab90953 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Jurkat cells, Hela, 3T3 NIH, cos1, Testis, ES cell)
Loading amount
50 µg
Specification
Jurkat cells, Hela, 3T3 NIH, cos1, Testis, ES cell
Gel Running Conditions
Reduced Denaturing (SDS-PAGE gel 12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 4% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 10 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (16 dpp testes)
Specification
16 dpp testes
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 04 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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