Overview

  • Product name

    Anti-Tex19.1 antibody
  • Description

    Rabbit polyclonal to Tex19.1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Tex19.1 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: Q99MV2

  • Positive control

    • This antibody gave a positive signal in both Mouse Testis and Placenta tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • Relevance

    The Human testis expressed gene 19 (Tex19) is duplicated in mouse and rat to give two genes Tex19.1 and Tex19.2. Tex19.1 expression is limited to pluripotent cells and germ cells, and is downregulated after differentiation. The Tex19.1 (-/-) knockout male mice have impaired spermatogenesis.
  • Database links

  • Alternative names

    • Testis expressed protein 19A antibody
    • Tex 19.1 antibody
    • Tex 19a antibody
    • Tex19a antibody

Images

  • All lanes : Anti-Tex19.1 antibody (ab129726) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Mouse Placenta Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 45 kDa (possible non-specific binding)


    Exposure time: 8 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129726 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab129726 has not yet been referenced specifically in any publications.

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