Texas Red ® Anti-Biotin antibody (ab6653)

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Western blot - Texas Red ® Anti-Biotin antibody (ab6653)

    Key features and details

    • Texas Red ® Goat polyclonal to Biotin
    • Suitable for: WB
    • Reacts with: Species independent
    • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
    • Isotype: IgG

    Overview

    • Product name

      Texas Red ® Anti-Biotin antibody
      See all Biotin primary antibodies

    • Description

      Texas Red ® Goat polyclonal to Biotin

    • Host species

      Goat

    • Conjugation

      Texas Red ®. Ex: 596nm, Em: 620nm

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Chemical/ Small Molecule conjugated to KLH.

    • General notes

      Texas Red™ is a registered trademark of Molecular Probes Inc.

      Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab6653 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent concentration.

    Target

    • Relevance

      Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

    • Alternative names

      • Vitamin B4 antibody

    Images

    • Western blot - Texas Red ® Anti-Biotin antibody (ab6653)
      Western blot - Texas Red ® Anti-Biotin antibody (ab6653)
      Texas Red ® Anti-Biotin antibody (ab6653) at 1/1000 dilution (60 minutes at room temperature) + Biotin conjugated Guinea Pig IgG separated by SDS-PAGE at 0.05 µg

      Observed band size: 25,55 kDa
      why is the actual band size different from the predicted?



      Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature.

    References (0)

    Publishing research using ab6653? Please let us know so that we can cite the reference in this datasheet.

    ab6653 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Our customer would like to know specifically what you used for the secondary as this primary is conjugated with Texas Red. Thanks again!

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    Answer

    There are still free epitopes available for a secondary antibody to bind. But you could also use an unconjugated secondary antibody.

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    Question

    Hi Jennifer Nice to talk with you. Please see the below answers attached to each questions what you had. Please reply ASAP.Thanks! Have a nice day! technical@abcam.com wrote: Dear Wenxin, I have some general questions, the answers to which will enable me to investigate this matter as quickly as possible. 1. Please describe the problem (high background, no staining etc). The particulate precipitate was observed from antibody-solution in the vial and also was found in the nodules section under confocal microscope. 2. On what material are you testing the antibody in IHC? •Species? Soybean (Glycine max) •Cell line? •Tissue? Nodules 3. How did you fix the samples? •Ethanol, methanol •Acetone •Paraformaldehyde and Other FAA 4. Did you apply antigen retrieval step? No •Enzymatic method •Heat mediated technique •Other 5. How did you block the unspecific binding sites? 3% BSA in TBST buffer 6. Primary antibody •Specification (in which species was it raised against)? Products was labeled with Biotin •At what dilution(s) have you tested this antibody? 1: 100 •Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 1-2 hr at RT; 2 x 5 min with TBST buffer 7. Secondary antibody NO •What secondary antibody are you using? •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation, wash steps? •Do you know whether the problems you are experiencing come from the secondary? •What detection method are you using? 8. Background staining •Please provide an image of your staining we have no result for this experiment for several times but we have very nice data when we use Dig-system. 9. Which detection system did you use? confocal microscope 10. Did you apply positive and negative controls along with the samples? Please specify. Yes, no products were generated under negative control. For positive, we used biotin-anti--Dig. 11. Optimization attempts •How many times have you tried the IHC? Three times for each fixed sample (FAA or Paraformaldehyde) •Do you obtain the same results every time? Yes •What steps have you altered? No

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    Answer

    Thank you very much for the information that you have provided. You should not have precipitate in your vial and so I'm sending you a replacement vial free of charge. For your record it is order# 39078 and you should receive it Tuesday or Wednesday of next week. Please let me know how the replacement works out for you.

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    Question

    Hello, Can you tell me how many dyes (texas Red) are attached per molecule of anti-biotin antibody? Waiting for your reply and thanking you in advance. Cheers.

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    Answer

    The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

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