Key features and details
- Texas Red ® Goat polyclonal to Biotin
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Isotype: IgG
Product nameTexas Red ® Anti-Biotin antibody
See all Biotin primary antibodies
DescriptionTexas Red ® Goat polyclonal to Biotin
ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule conjugated to KLH.
Texas Red™ is a registered trademark of Molecular Probes Inc.
Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
Our Abpromise guarantee covers the use of ab6653 in the following tested applications.
|WB||Use at an assay dependent concentration.|
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
Texas Red ® Anti-Biotin antibody (ab6653) at 1/1000 dilution (60 minutes at room temperature) + Biotin conjugated Guinea Pig IgG separated by SDS-PAGE at 0.05 µg
Observed band size: 25,55 kDa why is the actual band size different from the predicted?
Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature.
ab6653 has not yet been referenced specifically in any publications.