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    texas-red--r-phycoerythrinrpea-antibody-ab34734.pdf

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Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)

  • Datasheet
Submit a review Q&A (1)References (1)

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Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)

    Key features and details

    • Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA
    • Suitable for: WB
    • Reacts with: Species independent
    • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
    • Isotype: IgG

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    Overview

    • Product name

      Texas Red ® Anti-R Phycoerythrin/rpeA antibody
      See all R Phycoerythrin/rpeA primary antibodies
    • Description

      Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA
    • Host species

      Goat
    • Conjugation

      Texas Red ®. Ex: 596nm, Em: 620nm
    • Specificity

      Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin/rpeA conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Other Immunogen Type corresponding to R Phycoerythrin/rpeA.

    • General notes

      Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.

       This product was previously labelled as R Phycoerythrin

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified by immunoaffinity chromatography using R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Conjugates
      • Cell Biology
      • Other Antibodies
      • Plant related targets

    Associated products

      Applications

      Our Abpromise guarantee covers the use of ab34734 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      WB Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.

      Target

      • Relevance

        Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
      • Cellular localization

        Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
      • Alternative names

        • R phycoerythrin alpha subunit antibody
        • R phycoerythrin beta subunit antibody
        • rpeA antibody
        • rpeB antibody

      Images

      • Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)
        Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)
        Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734) at 1/1000 dilution (60 minutes at room temperature) + R-Phycoerythrin at 0.05 µg

        Predicted band size: 18 kDa
        Observed band size: 18 kDa



        Blocking buffer for fluorescent Western blotting used for 30 minutes at room temperature.
        Predicted size: 18 kDa for R-Phycoerythrin.
        Other bands: R-Phycoerythrin splice varients and isoforms.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab34734? Please let us know so that we can cite the reference in this datasheet.

      ab34734 has been referenced in 1 publication.

      • Andersen TK  et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines 4:9 (2019). PubMed: 30775000

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Is "R-PE" the same thing as PE?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 23 2012

      Answer

      Thank you for contacting us.

      R-PE is a variety of PE, (B-PE is another), so it is correct to say that R-PE is PE, but so is B-PE.

      If you are trying to detect a PE conjugate, ab34734 is appropriate, as it will detect B-PE as well as R-PE. It should really be considered "anti-PE", but is named "anti-R-PE" because the immunogen that generated the antibody was R-PE.

      I hope this helps. If you have any other questions, please contact us.

      Read More

      Abcam Scientific Support

      Answered on Mar 23 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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