Key features and details
- Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Isotype: IgG
Product nameTexas Red ® Anti-R Phycoerythrin/rpeA antibody
See all R Phycoerythrin/rpeA primary antibodies
DescriptionTexas Red ® Goat polyclonal to R Phycoerythrin/rpeA
ConjugationTexas Red ®. Ex: 596nm, Em: 620nm
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin/rpeA conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Other Immunogen Type corresponding to R Phycoerythrin/rpeA.
Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.
This product was previously labelled as R Phycoerythrin
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by immunoaffinity chromatography using R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Our Abpromise guarantee covers the use of ab34734 in the following tested applications.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.|
RelevancePhycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
Cellular localizationPlastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734) at 1/1000 dilution (60 minutes at room temperature) + R-Phycoerythrin at 0.05 µg
Predicted band size: 18 kDa
Observed band size: 18 kDa
Blocking buffer for fluorescent Western blotting used for 30 minutes at room temperature.
Predicted size: 18 kDa for R-Phycoerythrin.
Other bands: R-Phycoerythrin splice varients and isoforms.
ab34734 has been referenced in 1 publication.
- Andersen TK et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines 4:9 (2019). PubMed: 30775000