Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)

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Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)

    Key features and details

    • Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA
    • Suitable for: WB
    • Reacts with: Species independent
    • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
    • Isotype: IgG

    Overview

    • Product name

      Texas Red ® Anti-R Phycoerythrin/rpeA antibody
      See all R Phycoerythrin/rpeA primary antibodies

    • Description

      Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA

    • Host species

      Goat

    • Conjugation

      Texas Red ®. Ex: 596nm, Em: 620nm

    • Specificity

      Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin/rpeA conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Other Immunogen Type corresponding to R Phycoerythrin/rpeA.

    • General notes

      Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.

       This product was previously labelled as R Phycoerythrin

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Purified by immunoaffinity chromatography using R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab34734 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.

    Target

    • Relevance

      Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.

    • Cellular localization

      Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.

    • Alternative names

      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

    Images

    • Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)
      Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)
      Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734) at 1/1000 dilution (60 minutes at room temperature) + R-Phycoerythrin at 0.05 µg

      Predicted band size: 18 kDa
      Observed band size: 18 kDa



      Blocking buffer for fluorescent Western blotting used for 30 minutes at room temperature.
      Predicted size: 18 kDa for R-Phycoerythrin.
      Other bands: R-Phycoerythrin splice varients and isoforms.

    References (1)

    Publishing research using ab34734? Please let us know so that we can cite the reference in this datasheet.

    ab34734 has been referenced in 1 publication.

    • Andersen TK  et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines 4:9 (2019). PubMed: 30775000

    Customer reviews and Q&As

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    Question

    Is "R-PE" the same thing as PE?

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    Answer

    Thank you for contacting us.

    R-PE is a variety of PE, (B-PE is another), so it is correct to say that R-PE is PE, but so is B-PE.

    If you are trying to detect a PE conjugate, ab34734 is appropriate, as it will detect B-PE as well as R-PE. It should really be considered "anti-PE", but is named "anti-R-PE" because the immunogen that generated the antibody was R-PE.

    I hope this helps. If you have any other questions, please contact us.

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