Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)
Key features and details
- Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Isotype: IgG
Overview
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Product name
Texas Red ® Anti-R Phycoerythrin/rpeA antibody
See all R Phycoerythrin/rpeA primary antibodies -
Description
Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA -
Host species
Goat -
Conjugation
Texas Red ®. Ex: 596nm, Em: 620nm -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin/rpeA conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length protein corresponding to R Phycoerythrin/rpeA. This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
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General notes
Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by immunoaffinity chromatography using R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa. |
Target
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Relevance
Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. -
Cellular localization
Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome. -
Alternative names
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
Images
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Western blot - Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734)Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734) at 1/1000 dilution (60 minutes at room temperature) + R-Phycoerythrin at 0.05 µg
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking buffer for fluorescent Western blotting used for 30 minutes at room temperature.
Predicted size: 18 kDa for R-Phycoerythrin.
Other bands: R-Phycoerythrin splice varients and isoforms.
Datasheets and documents
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Datasheet download
References (1)
ab34734 has been referenced in 1 publication.
- Andersen TK et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines 4:9 (2019). PubMed: 30775000
