Anti-TFAM antibody (ab131607)

Rabbit polyclonal TFAM antibody. Validated in WB and tested in Mouse, Rat, Human. Cited in 35 publication(s). Independently reviewed in 9 review(s).

Overview

  • Product name

  • Description

    Rabbit polyclonal to TFAM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Native recombinant Mouse mtTFA protein with a C-terminal 6-His tag.

  • Positive control

    • Rat kidney lysate.

Applications

Our Abpromise guarantee covers the use of ab131607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 28 kDa.

Images

  • All lanes : Anti-TFAM antibody (ab131607) at 1/2000 dilution

    Lane 1 : Liver
    Lane 2 : Hepatocytes

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa


    Exposure time: 10 seconds


    ab131607 at 1/1000 detecting TFAM from mouse liver and hepatocytes (whole cell) (25ug/lane) by Western Blot. An HRP conjugated goat anti-rabbit IgG was used as the secondary and ECL was used as the detection method.

    See Abreview

  • Anti-TFAM antibody (ab131607) at 1/2000 dilution + Rat kidney lysate

    Predicted band size: 28 kDa

References

This product has been referenced in:

  • Pal S  et al. The osteogenic effect of liraglutide involves enhanced mitochondrial biogenesis in osteoblasts. Biochem Pharmacol 164:34-44 (2019). Read more (PubMed: 30885766) »
  • Zhao Y  et al. ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects. Nat Commun 10:1371 (2019). Read more (PubMed: 30914652) »
See all 50 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (retina)
Permeabilization
Yes - triton x-100
Specification
retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 10 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Oct 03 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 14 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Soleus muscle)
Gel Running Conditions
Reduced Denaturing (4-12% NuPAGE gel)
Loading amount
25 µg
Specification
Soleus muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Ms. Jenny Sharpe

Verified customer

Submitted Jun 29 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Apr 14 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Hela, Hep G2)
Specification
Hela, Hep G2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 06 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (mouse heart)
Specification
mouse heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Nov 03 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (H9C2)
Specification
H9C2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Nov 03 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Cynomolgus Monkey Tissue lysate - whole (Peripheral Blood Mononuclear Cell)
Specification
Peripheral Blood Mononuclear Cell
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Daniel Tyrrell

Verified customer

Submitted Sep 30 2013

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