Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab175081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 38 kDa.

Target

  • Function
    Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating enhancer binding protein 4 antibody
    • Activating enhancer-binding protein 4 antibody
    • AP 4 antibody
    • AP4 antibody
    • bHLHc41 antibody
    • Class C basic helix-loop-helix protein 41 antibody
    • TFAP 4 antibody
    • TFAP4 antibody
    • TFAP4 protein antibody
    • TFAP4_HUMAN antibody
    • Transcription factor AP 4 antibody
    • Transcription factor AP-4 antibody
    • Transcription factor AP4 antibody
    see all

Images

  • Anti-TFAP4 antibody - C-terminal (ab175081) at 1/100 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size: 38 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TFAP4 with ab175081 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometric analysis of Jurkat cells labeling TFAP4 with ab175081 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175081 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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