Overview

  • Product name
    Anti-TFB2M antibody
    See all TFB2M primary antibodies
  • Description
    Goat polyclonal to TFB2M
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    RNLVRDLLEHQNPS

    with a Cysteine residue linker, corresponding to internal sequence amino acids 81-94 of Mouse TFB2M (NP_032275.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Mouse and Rat lung lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab118321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
    • Alternative names
      • N''-adenosyl(rRNA) dimethyltransferase 2 antibody
      • S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2 antibody
      • Dimethyladenosine transferase 2 antibody
      • dimethyladenosine transferase 2 mitochondrial antibody
      • FLJ22661 antibody
      • FLJ23182 antibody
      • h mtTFB antibody
      • h mtTFB2 antibody
      • h-mtTFB antibody
      • h-mtTFB2 antibody
      • HCV NS5A transactivated protein 5 antibody
      • HCV NS5A-transactivated protein 5 antibody
      • hepatitis C virus NS5A transactivated protein 5 antibody
      • Hepatitis C virus NS5A-transactivated protein 5 antibody
      • Hkp1 antibody
      • hTFB2M antibody
      • Mitochondrial 12S rRNA dimethylase 2 antibody
      • mitochondrial antibody
      • Mitochondrial dimethyladenosine transferase 2 antibody
      • Mitochondrial transcription factor B2 antibody
      • mtTFB antibody
      • mtTFB2 antibody
      • NS5ATP5 antibody
      • S-adenosylmethionine-6-N'' antibody
      • TFB2M antibody
      • TFB2M_HUMAN antibody
      • Transcription Factor B2 antibody
      • Transcription factor B2 mitochondrial antibody
      see all

    Images

    • Western blot - Anti-TFB2M antibody (ab118321)
      Western blot - Anti-TFB2M antibody (ab118321)
      Anti-TFB2M antibody (ab118321) at 0.5 µg/ml + Rat Lung lysate (in RIPA buffer) at 35 µg

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 37 kDa (why is the actual band size different from the predicted?)



      The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.

    References

    ab118321 has not yet been referenced specifically in any publications.

    Publishing research using ab118321? Please let us know so that we can cite the reference in this datasheet.

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