Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab118321 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Our Abpromise guarantee covers the use of ab118321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).|
FunctionS-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
- Information by UniProt
- N''-adenosyl(rRNA) dimethyltransferase 2 antibody
- S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2 antibody
- Dimethyladenosine transferase 2 antibody
Anti-TFB2M antibody (ab118321) at 0.5 µg/ml + Rat Lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
ab118321 has not yet been referenced specifically in any publications.