Anti-TFB2M antibody (ab118321)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TFB2M antibody
See all TFB2M primary antibodies -
Description
Goat polyclonal to TFB2M -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide:
RNLVRDLLEHQNPS
with a Cysteine residue linker, corresponding to internal sequence amino acids 81-94 of Mouse TFB2M (NP_032275.2). -
Positive control
- Mouse and Rat lung lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab118321 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab118321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 15278 Mouse
- Entrez Gene: 289307 Rat
- SwissProt: Q3TL26 Mouse
- SwissProt: Q5U2T7 Rat
- Unigene: 293529 Mouse
- Unigene: 96104 Rat
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Alternative names
- N''-adenosyl(rRNA) dimethyltransferase 2 antibody
- S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2 antibody
- Dimethyladenosine transferase 2 antibody
see all
Images
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Western blot - Anti-TFB2M antibody (ab118321)Anti-TFB2M antibody (ab118321) at 0.5 µg/ml + Rat Lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
Protocols
Datasheets and documents
References
ab118321 has not yet been referenced specifically in any publications.
