Key features and details
- Rabbit polyclonal to TFE3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TFE3 antibody
See all TFE3 primary antibodies
DescriptionRabbit polyclonal to TFE3
Specificityab196681 detects endogenous levels of total TFE3 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human TFE3 (internal sequence). The exact sequence is proprietary.
Database link: P19532
- K562 and A549 cell extracts. Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/3000. Predicted molecular weight: 62 kDa.|
FunctionTranscription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
Tissue specificityUbiquitous in fetal and adult tissues.
Involvement in diseaseNote=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
Sequence similaritiesBelongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
modificationsSumoylated; does not affect dimerization with MITF.
- Information by UniProt
- bHLH e33 antibody
- bHLHe33 antibody
- Class E basic helix-loop-helix protein 33 antibody
All lanes : Anti-TFE3 antibody (ab196681) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : A549 cell extract
Lane 3 : A549 cell extract with immunizing peptide
Predicted band size: 62 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TFE3 with ab196681 at 1/50 dilution. The image on the right is preincubated with blocking peptide.
ab196681 has not yet been referenced specifically in any publications.