• Product name

  • Description

    Rabbit polyclonal to TFE3
  • Host species

  • Specificity

    ab196681 detects endogenous levels of total TFE3 protein.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human TFE3 (internal sequence). The exact sequence is proprietary.
    Database link: P19532

  • Positive control

    • K562 and A549 cell extracts. Human brain tissue.



Our Abpromise guarantee covers the use of ab196681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/3000. Predicted molecular weight: 62 kDa.


  • Function

    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
  • Tissue specificity

    Ubiquitous in fetal and adult tissues.
  • Involvement in disease

    Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
    Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
  • Sequence similarities

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational

    Sumoylated; does not affect dimerization with MITF.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • bHLH e33 antibody
    • bHLHe33 antibody
    • Class E basic helix-loop-helix protein 33 antibody
    • RCCP 2 antibody
    • RCCP2 antibody
    • RCCX1 antibody
    • Renal cell carcinoma, papillary antibody
    • Tcfe 3 antibody
    • Tcfe3 antibody
    • TFE 3 antibody
    • Tfe3 antibody
    • TFE3_HUMAN antibody
    • TFEA antibody
    • Transcription factor binding to IGHM enhancer 3 antibody
    • Transcription factor E family, member A antibody
    • Transcription factor E3 antibody
    • Transcription factor for IgH enhancer antibody
    • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
    see all


  • All lanes : Anti-TFE3 antibody (ab196681) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : A549 cell extract
    Lane 3 : A549 cell extract with immunizing peptide

    Predicted band size: 62 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TFE3 with ab196681 at 1/50 dilution. The image on the right is preincubated with blocking peptide.


ab196681 has not yet been referenced specifically in any publications.

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