Product nameAnti-TFE3 antibody
See all TFE3 primary antibodies
DescriptionChicken polyclonal to TFE3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to amino acids 432-541 of Human TFE3
- E. coli-derived TFE3 fusion protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: None
Constituents: PBS, pH 7.6
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).|
FunctionTranscription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
Tissue specificityUbiquitous in fetal and adult tissues.
Involvement in diseaseNote=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
Sequence similaritiesBelongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
modificationsSumoylated; does not affect dimerization with MITF.
- Information by UniProt
- bHLH e33 antibody
- bHLHe33 antibody
- Class E basic helix-loop-helix protein 33 antibody
Anti-TFE3 antibody (ab36642) at 1/2000 dilution + E. coli-derived TFE3 fusion protein at 2.5 µg
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size: 80 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
This protein is expected to be 74 kDa, but the protein fragment is from amino acids 432-541, or about 12 kDa. This is a fusion protein with a partner of 52 kDa which totals 64 kDa, shown on the blot.
ab36642 has not yet been referenced specifically in any publications.