Product nameAnti-TFE3 antibody
See all TFE3 primary antibodies
DescriptionRabbit polyclonal to TFE3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
Synthetic peptide from a region within amino acids 500-550 of Human TFE3 (NP_006512.2)
- HeLa and 293T cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
Tissue specificityUbiquitous in fetal and adult tissues.
Involvement in diseaseNote=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
Sequence similaritiesBelongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
modificationsSumoylated; does not affect dimerization with MITF.
- Information by UniProt
- bHLH e33 antibody
- bHLHe33 antibody
- Class E basic helix-loop-helix protein 33 antibody
All lanes : Anti-TFE3 antibody (ab93808) at 0.04 µg/ml
Lane 1 : HeLa whole lysate at 50 µg
Lane 2 : HeLa whole lysate at 15 µg
Lane 3 : HeLa whole lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 3 minutes
1 mg HeLa cell lysate was immunoprecipitated with 3µg/mg ab93808 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitates were analysed by Western blot. Bands were analysed using ab93808 at 0.4µg/ml and developed using cheiluminescence.
This product has been referenced in:
- Yin X et al. TFE3 fusions escape from controlling of mTOR signaling pathway and accumulate in the nucleus promoting genes expression in Xp11.2 translocation renal cell carcinomas. J Exp Clin Cancer Res 38:119 (2019). Read more (PubMed: 30849994) »
- Saj A et al. Disrupting Interactions Between ß-Catenin and Activating TCFs Reconstitutes Ground State Pluripotency in Mouse Embryonic Stem Cells. Stem Cells 35:1924-1933 (2017). Read more (PubMed: 28577307) »