Anti-TFE3 antibody (ab93808)
Key features and details
- Rabbit polyclonal to TFE3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFE3 antibody
See all TFE3 primary antibodies -
Description
Rabbit polyclonal to TFE3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant -
Immunogen
Synthetic peptide from a region within amino acids 500-550 of Human TFE3 (NP_006512.2)
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Positive control
- HeLa and 293T cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
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IP |
Use a concentration of 2 - 5 µg/ml.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 62 kDa. |
IP
Use a concentration of 2 - 5 µg/ml. |
Target
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Function
Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. -
Tissue specificity
Ubiquitous in fetal and adult tissues. -
Involvement in disease
Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. -
Sequence similarities
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsSumoylated; does not affect dimerization with MITF. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7030 Human
- Entrez Gene: 209446 Mouse
- Entrez Gene: 317376 Rat
- Omim: 314310 Human
- SwissProt: Q05B92 Cow
- SwissProt: P19532 Human
- SwissProt: Q64092 Mouse
- Unigene: 249142 Mouse
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Alternative names
- bHLH e33 antibody
- bHLHe33 antibody
- Class E basic helix-loop-helix protein 33 antibody
see all
Images
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All lanes : Anti-TFE3 antibody (ab93808) at 0.04 µg/ml
Lane 1 : HeLa whole lysate at 50 µg
Lane 2 : HeLa whole lysate at 15 µg
Lane 3 : HeLa whole lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 3 minutes -
1 mg HeLa cell lysate was immunoprecipitated with 3µg/mg ab93808 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitates were analysed by Western blot. Bands were analysed using ab93808 at 0.4µg/ml and developed using cheiluminescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab93808 has been referenced in 8 publications.
- Tian AL et al. Lysosomotropic agents including azithromycin, chloroquine and hydroxychloroquine activate the integrated stress response. Cell Death Dis 12:6 (2021). PubMed: 33414432
- Tan A et al. TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis. Cell Death Dis 12:343 (2021). PubMed: 33795648
- He X et al. TFE3-Mediated Autophagy is Involved in Dopaminergic Neurodegeneration in Parkinson's Disease. Front Cell Dev Biol 9:761773 (2021). PubMed: 34912803
- Wu Q et al. Isobacachalcone induces autophagy and improves the outcome of immunogenic chemotherapy. Cell Death Dis 11:1015 (2020). PubMed: 33243998
- Wang Y et al. Autophagy induction by thiostrepton improves the efficacy of immunogenic chemotherapy. J Immunother Cancer 8:N/A (2020). PubMed: 32221018
- Chen G et al. 3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB. EMBO Mol Med 11:e10469 (2019). PubMed: 31609086
- Yin X et al. TFE3 fusions escape from controlling of mTOR signaling pathway and accumulate in the nucleus promoting genes expression in Xp11.2 translocation renal cell carcinomas. J Exp Clin Cancer Res 38:119 (2019). PubMed: 30849994
- Saj A et al. Disrupting Interactions Between ß-Catenin and Activating TCFs Reconstitutes Ground State Pluripotency in Mouse Embryonic Stem Cells. Stem Cells 35:1924-1933 (2017). PubMed: 28577307