Overview

  • Product name

    Anti-TFE3 antibody [EPR11591]
    See all TFE3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11591] to TFE3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TFE3. The exact sequence is proprietary.
    Database link: P19532

  • Positive control

    • Human renal cell carcinoma and thyroid gland carcinoma tissues; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
    • Tissue specificity

      Ubiquitous in fetal and adult tissues.
    • Involvement in disease

      Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
      Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
    • Sequence similarities

      Belongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications

      Sumoylated; does not affect dimerization with MITF.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • bHLH e33 antibody
      • bHLHe33 antibody
      • Class E basic helix-loop-helix protein 33 antibody
      • RCCP 2 antibody
      • RCCP2 antibody
      • RCCX1 antibody
      • Renal cell carcinoma, papillary antibody
      • Tcfe 3 antibody
      • Tcfe3 antibody
      • TFE 3 antibody
      • Tfe3 antibody
      • TFE3_HUMAN antibody
      • TFEA antibody
      • Transcription factor binding to IGHM enhancer 3 antibody
      • Transcription factor E family, member A antibody
      • Transcription factor E3 antibody
      • Transcription factor for IgH enhancer antibody
      • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling TFE3 with Purified ab179804 at 1:100 dilution (6.59 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling TFE3 with unpurified ab179804 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human renal cell carcinoma tissue labeling TFE3 with unpurified ab179804 at 1/250 dilution.

    References

    This product has been referenced in:

    • Li S  et al. Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun 10:1693 (2019). Read more (PubMed: 30979895) »
    • Sun Q  et al. Diagnosis and treatment of a carotid body tumor: A case report of a rare bilateral tumor. Oncol Lett 14:6417-6420 (2017). Read more (PubMed: 29375704) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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