Recombinant
RabMAb

Recombinant Anti-TFE3 antibody [EPR11591] - BSA and Azide free (ab231112)

Overview

  • Product name

    Anti-TFE3 antibody [EPR11591] - BSA and Azide free
    See all TFE3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11591] to TFE3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. The exact sequence is proprietary.
    Database link: P19532

  • Positive control

    • Human renal cell carcinoma and thyroid gland carcinoma tissues; HeLa cells.
  • General notes

    ab231112 is the carrier-free version of ab179804 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab231112 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab231112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
    • Tissue specificity

      Ubiquitous in fetal and adult tissues.
    • Involvement in disease

      Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
      Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
    • Sequence similarities

      Belongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications

      Sumoylated; does not affect dimerization with MITF.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • bHLH e33 antibody
      • bHLHe33 antibody
      • Class E basic helix-loop-helix protein 33 antibody
      • RCCP 2 antibody
      • RCCP2 antibody
      • RCCX1 antibody
      • Renal cell carcinoma, papillary antibody
      • Tcfe 3 antibody
      • Tcfe3 antibody
      • TFE 3 antibody
      • Tfe3 antibody
      • TFE3_HUMAN antibody
      • TFEA antibody
      • Transcription factor binding to IGHM enhancer 3 antibody
      • Transcription factor E family, member A antibody
      • Transcription factor E3 antibody
      • Transcription factor for IgH enhancer antibody
      • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling TFE3 with Purified ab179804 at 1:100 dilution (6.59 μg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179804).

    • This IHC data was generated using the same anti-TFE3 antibody clone, EPR11591, in a different buffer formulation (cat# ab179804).

       Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling TFE3 with unpurified ab179804 at 1/250 dilution.

    • This IHC data was generated using the same anti-TFE3 antibody clone, EPR11591, in a different buffer formulation (cat# ab179804).

      Immunohistochemical analysis of paraffin-embedded Human renal cell carcinoma tissue labeling TFE3 with unpurified ab179804 at 1/250 dilution.

    References

    ab231112 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab231112.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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