Overview

  • Product name

  • Description

    Rabbit polyclonal to TFEB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TFEB aa 325-375. The exact sequence is proprietary. NP_009093.1
    Database link: P19484

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 53 kDa.

Target

  • Function

    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
  • Sequence similarities

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.
  • Post-translational
    modifications

    Sumoylated; does not affect dimerization with MITF.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha TFEB antibody
    • AlphaTFEB antibody
    • bHLHe35 antibody
    • Class E basic helix-loop-helix protein 35 antibody
    • T cell transcription factor EB antibody
    • TCFEB antibody
    • TFEB antibody
    • TFEB_HUMAN antibody
    • Transcription factor EB antibody
    see all

Images

  • All lanes : Anti-TFEB antibody (ab245349) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 53 kDa


    Exposure time: 10 seconds


    Prepared using NETN lysis buffer.

  • TFEB was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    ab245349 used for IP at 6 µg per reaction. For WB 1 µg/ml.

    Lane 1: ab245349 (Lot 2) IP in HeLa whole cell lysate.

    Lane 2: ab245349 (Lot 3) IP in HeLa whole cell lysate.

    Lane 3: Control IgG.

    Chemiluminescence detection: 10 seconds.

References

ab245349 has not yet been referenced specifically in any publications.

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