Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab82143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
    • Sequence similarities

      Belongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Domain

      The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.
    • Post-translational
      modifications

      Sumoylated; does not affect dimerization with MITF.
    • Cellular localization

      Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha TFEB antibody
      • AlphaTFEB antibody
      • bHLHe35 antibody
      • Class E basic helix-loop-helix protein 35 antibody
      • T cell transcription factor EB antibody
      • TCFEB antibody
      • TFEB antibody
      • TFEB_HUMAN antibody
      • Transcription factor EB antibody
      see all

    Images

    • Anti-TFEB antibody (ab82143) at 1 µg/ml + human lung lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 53 kDa
      Observed band size: 53 kDa



      Primary incubation was 1 hour.

    References

    This product has been referenced in:

    • Liu Y  et al. KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway. Oncotarget 7:53153-53164 (2016). Read more (PubMed: 27449290) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse Schwann cell)
    Loading amount
    20 µg
    Specification
    mouse Schwann cell
    Treatment
    starvation 8 hours
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Ms. Jessica Nicks

    Verified customer

    Submitted Jan 29 2013

    Answer


    I see that you mentioned in the additional notes that there was a very faint band at 43kDa. I just wanted to inform you that the expected band size for TFEB is 53kDa. Did you see a positive band around the 53dDa size?
    I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased within the last six months, we are happy to provide scientific support and to replace or refund the product should we not be able to help.
    Upon initial review of your protocol, I have a few recommendations to potentially reduce the number of non-specific bands you are seeing:
    1. Increase the blocking time
    2. Include protease and phosphatase inhibitors (TFEB is undergoes post-translational phosphorylation)
    Further troubleshooting tips are given through the link below:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11352#3

    Read More

    Question
    Answer

    Thank you for your phone call.

    I have arranged for the free of charge replacement with the human lung tissue lysate ab30281. The order number is xxx and you should receive it tomorrow.

    Please let me know if you have further questions. I'd be happy to help.


    Here is the information regarding your testing discount and our Abtrial Program:

    DISCOUNT CODE: xxx
    Expiration date: xxx
    Value: xxx

    I am very pleased to hear you would like to accept our offer and test ab82143 in mouse. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

    Read More

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