Anti-TFEB antibody - ChIP Grade (ab2636)

Goat polyclonal TFEB antibody. Validated in WB, IHC, ChIP and tested in Human. Cited in 24 publication(s). Independently reviewed in 4 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-TFEB antibody - ChIP Grade
    See all TFEB primary antibodies
  • Description

    Goat polyclonal to TFEB - ChIP Grade
  • Host species

    Goat
  • Specificity

    This antibody is raised against TCFEB. However a customer reports that this antibody cross reacts with mouse TFE3 and MITF (other members of transcription factor family Mi/Tfe) in over-expressing cell lines.
  • Tested applications

    Suitable for: WB, ChIPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chinese hamster
  • Immunogen

    Synthetic peptide:

    ASRIGLRMQLMRE

    , corresponding to N terminal amino acids 2-14 of Human TFEB.

  • Positive control

  • General notes

    Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody

    Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)  

Properties

Applications

Our Abpromise guarantee covers the use of ab2636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml.

Primary incubation 1 hour at room temperature. 

Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively. Preliminary testing was unsuccessful on cell line NIH3T3 for this particular batch.

 

ChIP Use at an assay dependent concentration. PubMed: 17085443

Target

  • Function

    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
  • Sequence similarities

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.
  • Post-translational
    modifications

    Sumoylated; does not affect dimerization with MITF.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha TFEB antibody
    • AlphaTFEB antibody
    • bHLHe35 antibody
    • Class E basic helix-loop-helix protein 35 antibody
    • T cell transcription factor EB antibody
    • TCFEB antibody
    • TFEB antibody
    • TFEB_HUMAN antibody
    • Transcription factor EB antibody
    see all

Images

  • Lane 1 : Anti-TFEB antibody - ChIP Grade (ab2636) at 0.5 µg/ml
    Lane 2 : Anti-TFEB antibody - ChIP Grade (ab2636) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate


    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-TFEB antibody - ChIP Grade (ab2636) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µg

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:

  • Liu N  et al. Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2a-mediated tumor progression. J Clin Invest N/A:N/A (2019). Read more (PubMed: 30431439) »
  • Magini A  et al. Curcumin Analogue C1 Promotes Hex and Gal Recruitment to the Plasma Membrane via mTORC1-Independent TFEB Activation. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30889901) »
See all 26 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEFs)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Jul 04 2018

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Insu Kwon

Verified customer

Submitted Dec 10 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Brain
Blocking step
Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 22 2015

Answer

Thank you for your inquiry.

We do not batch test this antibody for ChIP in-house, but this antibody has been successfully used in ChIP as described in the following papers:

Esumi N et al. VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family. J Biol Chem 282:1838-50 (2007). PMID: 17085443.

Huan C et al. Renal Carcinoma-associated Transcription Factors TFE3 and TFEB Are Leukemia Inhibitory Factor-responsive Transcription Activators of E-cadherin. J Biol Chem 280:30225-35 (2005). PubMed: 15994295

We would guarantee this antibody to work in ChIP in accordance with our Abpromise.

I hope this information helps. Please contact us with any other questions.

Read More
Application
Western blot
Sample
Human Cell lysate - nuclear (breast cancer cell lines)
Loading amount
40 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing (8 % acrylamide gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 01 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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