Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14871] to TFEC
- Suitable for: IP, WB
- Reacts with: Rat, Human
Product nameAnti-TFEC antibody [EPR14871]
See all TFEC primary antibodies
DescriptionRabbit monoclonal [EPR14871] to TFEC
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human TFEC aa 200-300. The exact sequence is proprietary.
Database link: O14948
- 293T and HepG2 whole cell lysate (ab7900).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/40 - 1/60.|
|WB||1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).|
FunctionTranscriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter (By similarity). Collaborates with MITF in target gene activation (By similarity). Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence (By similarity). Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer (By similarity). Binds DNA in a homo- or heterodimeric form.
Tissue specificityExpressed moderately in spleen, kidney, bone marrow, small intestine and leukocytes. Expressed weakly in testis, trachea and colon.
Sequence similaritiesBelongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- bHLHe34 antibody
- Class E basic helix-loop-helix protein 34 antibody
- hTFEC-L antibody
All lanes : Anti-TFEC antibody [EPR14871] (ab185226) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Western blot analysis of immunoprecipitation pellet from 293T cell lysate immunoprecipitated using ab185226 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
ab185226 has not yet been referenced specifically in any publications.