Overview

  • Product name

    Anti-TFEC antibody [EPR14871]
    See all TFEC primary antibodies
  • Description

    Rabbit monoclonal [EPR14871] to TFEC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human TFEC aa 200-300. The exact sequence is proprietary.
    Database link: O14948

  • Positive control

    • 293T and HepG2 whole cell lysate (ab7900).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40 - 1/60.
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter (By similarity). Collaborates with MITF in target gene activation (By similarity). Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence (By similarity). Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer (By similarity). Binds DNA in a homo- or heterodimeric form.
  • Tissue specificity

    Expressed moderately in spleen, kidney, bone marrow, small intestine and leukocytes. Expressed weakly in testis, trachea and colon.
  • Sequence similarities

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHe34 antibody
    • Class E basic helix-loop-helix protein 34 antibody
    • hTFEC-L antibody
    • TCFEC antibody
    • TFE-C antibody
    • Tfec antibody
    • TFEC protein antibody
    • TFEC_HUMAN antibody
    • TFECL antibody
    • Transcription factor EC antibody
    • Transcription factor EC-like antibody
    see all

Images

  • All lanes : Anti-TFEC antibody [EPR14871] (ab185226) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Western blot analysis of immunoprecipitation pellet from 293T cell lysate immunoprecipitated using ab185226 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab185226 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185226.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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