For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    tff2-antibody-epr63462-ab140645.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Other Antibodies Other Antibodies
Share by email
RecombinantRabMAb

Recombinant Anti-TFF2 antibody [EPR6346(2)] (ab140645)

  • Datasheet
  • SDS
Submit a review Q&A (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-TFF2 antibody [EPR6346(2)] (ab140645)
  • Anti-TFF2 antibody [EPR6346(2)] (ab140645)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6346(2)] to TFF2
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TFF2 antibody [EPR6346(2)]
    See all TFF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6346(2)] to TFF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TFF2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human stomach cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Spasmolytic Polypeptide

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6346(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Alternative Versions

    • Anti-TFF2 antibody [EPR6346(2)] - BSA and Azide free (ab248927)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant Human TFF2 protein (ab50085)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
    • Tissue specificity

      Stomach.
    • Sequence similarities

      Contains 2 P-type (trefoil) domains.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q03403 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7032 Human
      • Omim: 182590 Human
      • SwissProt: Q03403 Human
      • Unigene: 2979 Human
      • Alternative names

        • SML 1 antibody
        • SML1 antibody
        • SP antibody
        • Spasmolysin antibody
        • Spasmolytic polypeptide antibody
        • spasmolytic protein 1 antibody
        • TFF 2 antibody
        • TFF2 antibody
        • TFF2_HUMAN antibody
        • trefoil factor 2 (spasmolytic protein 1) antibody
        • Trefoil factor 2 antibody
        • Trefoil factor 2 precursor antibody
        see all

      Images

      • Western blot - Anti-TFF2 antibody [EPR6346(2)] (ab140645)
        Western blot - Anti-TFF2 antibody [EPR6346(2)] (ab140645)
        Anti-TFF2 antibody [EPR6346(2)] (ab140645) at 1/1000 dilution + Human stomach cell lysate at 10 µg

        Secondary
        HRP conjugated goat anti-rabbit at 1/2000 dilution

        Predicted band size: 14 kDa

      • Anti-TFF2 antibody [EPR6346(2)] (ab140645)
        Anti-TFF2 antibody [EPR6346(2)] (ab140645)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab140645? Please let us know so that we can cite the reference in this datasheet.

      ab140645 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Would you be so kind to tell me %homology of immunogenic peptide from hTff2 to mouse Tff2 peptide ?

      Read More

      Abcam community

      Verified customer

      Asked on Dec 03 2013

      Answer

      The immunogen for ab140645 has 86% homology with mouse. We have tested this antibody with mouse and it does not react. The only antibody to this target that we know works in mouse and would guarantee under our Abpromise is ab49536 in IHC-P.

      https://www.abcam.com/spasmolytic-polypeptide-antibody-ge16c-ab49536.html


      Mouse publications here: https://www.abcam.com/Spasmolytic-Polypeptide-antibody-GE16C-ab49536-references.html

      Read More

      Elena Valued Customer

      Abcam Scientific Support

      Answered on Dec 03 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.