RecombinantRabMAb

Recombinant Anti-TFF2 antibody [EPR6346(2)] (ab140645)

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Western blot - Anti-TFF2 antibody [EPR6346(2)] (ab140645)
  • Anti-TFF2 antibody [EPR6346(2)] (ab140645)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6346(2)] to TFF2
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TFF2 antibody [EPR6346(2)]
    See all TFF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6346(2)] to TFF2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human TFF2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human stomach cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Spasmolytic Polypeptide

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6346(2)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.

    • Tissue specificity

      Stomach.

    • Sequence similarities

      Contains 2 P-type (trefoil) domains.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • SML 1 antibody
        • SML1 antibody
        • SP antibody
        • Spasmolysin antibody
        • Spasmolytic polypeptide antibody
        • spasmolytic protein 1 antibody
        • TFF 2 antibody
        • TFF2 antibody
        • TFF2_HUMAN antibody
        • trefoil factor 2 (spasmolytic protein 1) antibody
        • Trefoil factor 2 antibody
        • Trefoil factor 2 precursor antibody
        see all

      Images

      • Western blot - Anti-TFF2 antibody [EPR6346(2)] (ab140645)
        Western blot - Anti-TFF2 antibody [EPR6346(2)] (ab140645)
        Anti-TFF2 antibody [EPR6346(2)] (ab140645) at 1/1000 dilution + Human stomach cell lysate at 10 µg

        Secondary
        HRP conjugated goat anti-rabbit at 1/2000 dilution

        Predicted band size: 14 kDa

      • Anti-TFF2 antibody [EPR6346(2)] (ab140645)
        Anti-TFF2 antibody [EPR6346(2)] (ab140645)

      References (0)

      Publishing research using ab140645? Please let us know so that we can cite the reference in this datasheet.

      ab140645 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Question

      Would you be so kind to tell me %homology of immunogenic peptide from hTff2 to mouse Tff2 peptide ?

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      Answer

      The immunogen for ab140645 has 86% homology with mouse. We have tested this antibody with mouse and it does not react. The only antibody to this target that we know works in mouse and would guarantee under our Abpromise is ab49536 in IHC-P.

      https://www.abcam.com/spasmolytic-polypeptide-antibody-ge16c-ab49536.html


      Mouse publications here: https://www.abcam.com/Spasmolytic-Polypeptide-antibody-GE16C-ab49536-references.html

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com