Anti-TFII I antibody (ab10464)
Key features and details
- Rabbit polyclonal to TFII I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFII I antibody
See all TFII I primary antibodies -
Description
Rabbit polyclonal to TFII I -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Immunogen was a synthetic peptide, which represented a portion of human general transcription factor II-I encoded within exons 27 and 28 (LocusLink ID 2969).
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Positive control
- Whole cell lysate from HEK293 cells treated with TFII-I siRNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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All applications |
Mouse
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 135 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 135 kDa). |
Target
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Function
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. -
Tissue specificity
Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues. -
Involvement in disease
Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Sequence similarities
Belongs to the TFII-I family.
Contains 6 GTF2I-like repeats. -
Post-translational
modificationsTransiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Sumoylated. -
Cellular localization
Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2969 Human
- Entrez Gene: 14886 Mouse
- Omim: 601679 Human
- SwissProt: P78347 Human
- SwissProt: Q9ESZ8 Mouse
- Unigene: 647041 Human
- Unigene: 261570 Mouse
- Unigene: 412191 Mouse
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Alternative names
- BAP 135 antibody
- BAP-135 antibody
- BAP135 antibody
see all
Images
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ab10464 detection of human TFII I by Western blot. Samples: Whole cell lysate (100
µ g) from HEK293 cells treated with TFII-I siRNA (+) or untreated (-). Antibody: ab10464 used at 0.2µ g/ml. Detection: Chemiluminescence. ab10464 detection of human TFII I by Western blot. Samples: Whole cell lysate (100 µg) from HEK293 cells treated with TFII-I siRNA (+) or untreated (-). Antibody: ab10464 used at 0.2 µg/ml. Detection: Chemiluminescence.
References (2)
ab10464 has been referenced in 2 publications.
- Narla ST et al. Activation of developmental nuclear fibroblast growth factor receptor 1 signaling and neurogenesis in adult brain by a7 nicotinic receptor agonist. Stem Cells Transl Med 2:776-88 (2013). PubMed: 24014683
- Crusselle-Davis VJ et al. Recruitment of coregulator complexes to the beta-globin gene locus by TFII-I and upstream stimulatory factor. FEBS J 274:6065-73 (2007). PubMed: 17970752