For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    tfii-i-antibody-ab10464.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription Other
Share by email

Anti-TFII I antibody (ab10464)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-TFII I antibody (ab10464)

    Key features and details

    • Rabbit polyclonal to TFII I
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-TFII I antibody
      See all TFII I primary antibodies
    • Description

      Rabbit polyclonal to TFII I
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Immunogen was a synthetic peptide, which represented a portion of human general transcription factor II-I encoded within exons 27 and 28 (LocusLink ID 2969).

    • Positive control

      • Whole cell lysate from HEK293 cells treated with TFII-I siRNA.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibodies were affinity purified using the peptide immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Polymerase associated factors
      • Pol II Transcription
      • Other
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Preinitiation complex

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human TFII I protein (ab152444)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10464 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Chimpanzee
    Rhesus monkey
    Gorilla
    Orangutan
    Application Abreviews Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 135 kDa).
    Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 135 kDa).

    Target

    • Function

      Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
    • Tissue specificity

      Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
    • Involvement in disease

      Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities

      Belongs to the TFII-I family.
      Contains 6 GTF2I-like repeats.
    • Post-translational
      modifications

      Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
      Sumoylated.
    • Cellular localization

      Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
    • Target information above from: UniProt accession P78347 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2969 Human
      • Entrez Gene: 14886 Mouse
      • Omim: 601679 Human
      • SwissProt: P78347 Human
      • SwissProt: Q9ESZ8 Mouse
      • Unigene: 647041 Human
      • Unigene: 261570 Mouse
      • Unigene: 412191 Mouse
      • Alternative names

        • BAP 135 antibody
        • BAP-135 antibody
        • BAP135 antibody
        • Bruton tyrosine kinase associated protein 135 antibody
        • Bruton tyrosine kinase-associated protein 135 antibody
        • BTK associated protein 135 antibody
        • BTK associated protein 135kD antibody
        • BTK associated protein antibody
        • BTK-associated protein 135 antibody
        • BTKAP 1 antibody
        • BTKAP1 antibody
        • DIWS antibody
        • FLJ38776 antibody
        • FLJ56355 antibody
        • General transcription factor II i antibody
        • General transcription factor II-I antibody
        • General transcription factor IIi antibody
        • GTF 2I antibody
        • Gtf2i antibody
        • GTF2I_HUMAN antibody
        • GTFII I antibody
        • GTFII-I antibody
        • IB 291 antibody
        • IB291 antibody
        • SPIN antibody
        • SRF Phox 1 interacting protein antibody
        • SRF Phox1 interacting protein antibody
        • SRF-Phox1-interacting protein antibody
        • TFII-I antibody
        • Transcription factor II I antibody
        • WBS antibody
        • WBSCR 6 antibody
        • WBSCR6 antibody
        • Williams Beuren syndrome chromosome region 6 antibody
        • Williams Beuren syndrome chromosome region 6 protein antibody
        • Williams-Beuren syndrome chromosomal region 6 protein antibody
        see all

      Images

      • Western blot - Anti-TFII I antibody (ab10464)
        Western blot - Anti-TFII I antibody (ab10464)
        ab10464 detection of human TFII I by Western blot. Samples: Whole cell lysate (100 µg) from HEK293 cells treated with TFII-I siRNA (+) or untreated (-). Antibody: ab10464 used at 0.2 µg/ml. Detection: Chemiluminescence. ab10464 detection of human TFII I by Western blot. Samples: Whole cell lysate (100 µg) from HEK293 cells treated with TFII-I siRNA (+) or untreated (-). Antibody: ab10464 used at 0.2 µg/ml. Detection: Chemiluminescence.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (2)

      Publishing research using ab10464? Please let us know so that we can cite the reference in this datasheet.

      ab10464 has been referenced in 2 publications.

      • Narla ST  et al. Activation of developmental nuclear fibroblast growth factor receptor 1 signaling and neurogenesis in adult brain by a7 nicotinic receptor agonist. Stem Cells Transl Med 2:776-88 (2013). PubMed: 24014683
      • Crusselle-Davis VJ  et al. Recruitment of coregulator complexes to the beta-globin gene locus by TFII-I and upstream stimulatory factor. FEBS J 274:6065-73 (2007). PubMed: 17970752

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab10464.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.