Product nameAnti-TFII I antibody
See all TFII I primary antibodies
DescriptionRabbit polyclonal to TFII I
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human TFII I aa 935-985. The exact sequence is proprietary. NP_127492.1.
Database link: P78347
- IHC-P: Human breast carcinoma tissue; Mouse teratoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 112 kDa.|
FunctionInteracts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
Tissue specificityUbiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
Involvement in diseaseNote=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the TFII-I family.
Contains 6 GTF2I-like repeats.
modificationsTransiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Cellular localizationCytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
- Information by UniProt
- BAP 135 antibody
- BAP-135 antibody
- BAP135 antibody
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for TFII I using ab264198 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
All lanes : Anti-TFII I antibody (ab264198) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Exposure time: 10 seconds
TFII I was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab264198 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitates using ab264198 1 µg/ml.
Lane 1: ab264198 IP in HeLa whole cell lysate.
Lane 1: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for TFII I using ab264198 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264198 has not yet been referenced specifically in any publications.