Overview

  • Product name

    Anti-TFII I antibody [EPR7697]
    See all TFII I primary antibodies
  • Description

    Rabbit monoclonal [EPR7697] to TFII I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human TFII I aa 950-1050. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, U937, and Jurkat lysates; Human colon tissue
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 135-138 kDa (predicted molecular weight: 112 kDa).
IHC-P 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
    • Tissue specificity

      Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
    • Involvement in disease

      Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Sequence similarities

      Belongs to the TFII-I family.
      Contains 6 GTF2I-like repeats.
    • Post-translational
      modifications

      Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
      Sumoylated.
    • Cellular localization

      Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • BAP 135 antibody
      • BAP-135 antibody
      • BAP135 antibody
      • Bruton tyrosine kinase associated protein 135 antibody
      • Bruton tyrosine kinase-associated protein 135 antibody
      • BTK associated protein 135 antibody
      • BTK associated protein 135kD antibody
      • BTK associated protein antibody
      • BTK-associated protein 135 antibody
      • BTKAP 1 antibody
      • BTKAP1 antibody
      • DIWS antibody
      • FLJ38776 antibody
      • FLJ56355 antibody
      • General transcription factor II i antibody
      • General transcription factor II-I antibody
      • General transcription factor IIi antibody
      • GTF 2I antibody
      • Gtf2i antibody
      • GTF2I_HUMAN antibody
      • GTFII I antibody
      • GTFII-I antibody
      • IB 291 antibody
      • IB291 antibody
      • SPIN antibody
      • SRF Phox 1 interacting protein antibody
      • SRF Phox1 interacting protein antibody
      • SRF-Phox1-interacting protein antibody
      • TFII-I antibody
      • Transcription factor II I antibody
      • WBS antibody
      • WBSCR 6 antibody
      • WBSCR6 antibody
      • Williams Beuren syndrome chromosome region 6 antibody
      • Williams Beuren syndrome chromosome region 6 protein antibody
      • Williams-Beuren syndrome chromosomal region 6 protein antibody
      see all

    Images

    • All lanes : Anti-TFII I antibody [EPR7697] (ab129025) at 1/10000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : U937 cell lysates
      Lane 4 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 112 kDa
      Observed band size: 135-138 kDa
      why is the actual band size different from the predicted?

    • ab129025, at 1/100 dilution staining TFII I in formalin fixed paraffin embedded Human colon tissue by immunohistochemistry
    • ab129025, at 1/100 dilution, staining TFII I in Hela cells by immunofluorescence
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Han SO  et al. Salmeterol with Liver Depot Gene Therapy Enhances the Skeletal Muscle Response in Murine Pompe Disease. Hum Gene Ther N/A:N/A (2019). Read more (PubMed: 30803275) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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