Key features and details
- Rabbit polyclonal to TFII I (phospho Y248)
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TFII I (phospho Y248) antibody
See all TFII I primary antibodies
DescriptionRabbit polyclonal to TFII I (phospho Y248)
Specificityab63344 detects endogenous levels of TFII I only when phosphorylated at tyrosine 248.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
synthesized phosphopeptide derived from human TFII I around the phosphorylation site of tyrosine 248 (P-D-YP-Y-Q).
- Extracts from LOVO cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab63344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).|
FunctionInteracts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
Tissue specificityUbiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
Involvement in diseaseNote=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the TFII-I family.
Contains 6 GTF2I-like repeats.
modificationsTransiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Cellular localizationCytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
- Information by UniProt
- BAP 135 antibody
- BAP-135 antibody
- BAP135 antibody
All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution
Lane 1 : extracts from LOVO cells, without immunising peptide
Lane 2 : extracts from LOVO cells, with immunising peptide
Predicted band size: 112 kDa
Observed band size: 112 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
ab63344 has not yet been referenced specifically in any publications.