For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    tfiib-acetyl-k238-antibody-ab5210.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIIB
Share by email

Anti-TFIIB (acetyl K238) antibody (ab5210)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.
  • Datasheet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

ELISA - Anti-TFIIB (acetyl K238) antibody (ab5210)
  • Immunocytochemistry/ Immunofluorescence - Anti-TFIIB (acetyl K238) antibody (ab5210)

Key features and details

  • Rabbit polyclonal to TFIIB (acetyl K238)
  • Suitable for: ELISA, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human TFIIB protein (ab81898)
Lysate
Product image
TFIIB overexpression 293T lysate (whole cell) (ab94156)

View more associated products

Overview

  • Product name

    Anti-TFIIB (acetyl K238) antibody
    See all TFIIB primary antibodies
  • Description

    Rabbit polyclonal to TFIIB (acetyl K238)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab26407)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIIB
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Preinitiation complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human TFIIB (acetyl K238) peptide (ab26407)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TFIIB protein (ab81898)

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.
Notes
ELISA
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
  • Sequence similarities

    Belongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q00403 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2959 Human
    • Entrez Gene: 229906 Mouse
    • Entrez Gene: 394390 Xenopus laevis
    • Entrez Gene: 324823 Zebrafish
    • Omim: 189963 Human
    • SwissProt: Q00403 Human
    • SwissProt: P62915 Mouse
    • SwissProt: P29054 Xenopus laevis
    • Unigene: 481852 Human
    • Unigene: 271756 Mouse
    • Unigene: 47842 Xenopus laevis
    see all
  • Alternative names

    • General transcription factor IIB antibody
    • General transcription factor TFIIB antibody
    • gtf2b antibody
    • RNA polymerase II transcription factor IIB antibody
    • S300 II antibody
    • S300-II antibody
    • TF IIB antibody
    • TF2B antibody
    • TF2B_HUMAN antibody
    • TFIIB antibody
    • Transcription initiation factor IIB antibody
    see all

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ELISA - Anti-TFIIB (acetyl K238) antibody (ab5210)
    ELISA - Anti-TFIIB (acetyl K238) antibody (ab5210)

    This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.

    Red line: ELISA against immunising peptide ab26407
    Green line: ELISA against non-modified peptide ab25428

  • Immunocytochemistry/ Immunofluorescence - Anti-TFIIB (acetyl K238) antibody (ab5210)
    Immunocytochemistry/ Immunofluorescence - Anti-TFIIB (acetyl K238) antibody (ab5210)
    ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab5210? Please let us know so that we can cite the reference in this datasheet.

ab5210 has been referenced in 1 publication.

  • Gelev V  et al. A new paradigm for transcription factor TFIIB functionality. Sci Rep 4:3664 (2014). WB ; Human . PubMed: 24441171

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab5210.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.