Anti-TFIIB (acetyl K238) antibody (ab5210)
Overview
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Product name
Anti-TFIIB (acetyl K238) antibody
See all TFIIB primary antibodies -
Description
Rabbit polyclonal to TFIIB (acetyl K238)This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Species reactivity
Predicted to work with: Mouse, Human, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human TFIIB aa 200-300 (intracellular) conjugated to keyhole limpet haemocyanin.
(Peptide available asab26407)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. -
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2959 Human
- Entrez Gene: 229906 Mouse
- Entrez Gene: 394390 Xenopus laevis
- Entrez Gene: 324823 Zebrafish
- Omim: 189963 Human
- SwissProt: Q00403 Human
- SwissProt: P62915 Mouse
- SwissProt: P29054 Xenopus laevis
see all -
Alternative names
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- gtf2b antibody
see all
Images
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ELISA - Anti-TFIIB (acetyl K238) antibody (ab5210)This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
Red line: ELISA against immunising peptide ab26407
Green line: ELISA against non-modified peptide ab25428 -
Immunocytochemistry/ Immunofluorescence - Anti-TFIIB (acetyl K238) antibody (ab5210)ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab5210 has been referenced in 1 publication.
- Gelev V et al. A new paradigm for transcription factor TFIIB functionality. Sci Rep 4:3664 (2014). WB ; Human . PubMed: 24441171
