Product nameAnti-TFIIB (acetyl K238) antibody
See all TFIIB primary antibodies
Predicted to work with: Mouse, Human, Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human TFIIB aa 200-300 (intracellular) conjugated to keyhole limpet haemocyanin.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionGeneral factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Sequence similaritiesBelongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
- Information by UniProt
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- gtf2b antibody
This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5210 has been referenced in 1 publication.
- Gelev V et al. A new paradigm for transcription factor TFIIB functionality. Sci Rep 4:3664 (2014). WB ; Human . PubMed: 24441171