Overview

  • Product name
    Anti-TFIIB antibody [EP4588]
    See all TFIIB primary antibodies
  • Description
    Rabbit monoclonal [EP4588] to TFIIB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TFIIB aa 50-150. The exact sequence is proprietary.

  • Positive control
    • WB: HepG2, HeLa, Jurkat and K562 cell lysates; IHC-P: Human breast tissue IP: Jurkat cells FC: Jurkat
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab109518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC.
  • Target

    Images

    • All lanes : Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

      Lane 1 : C6(Rat glial tumor glial cell) whole cell lysate
      Lane 2 : Raw264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 3 : PC-12(Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • ab109518 (purified) at 1/500 dilution (0.16 µg/ml) immunoprecipitating TFIIB in Jurkat whole cell lysate .
      Lane 1 (input): Jurkat(Human T cell leukemia T lymphocyte) whole cell lysate 10µg
      Lane 2 (+): ab109518 & Jurkat whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109518 in Jurkat whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM /TBST .

    • Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling TFIIB with ab109518 at 1/20 dilution (4.05 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
    • All lanes : Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution

      Lane 1 : HepG2 cell lyaste
      Lane 2 : HeLa cell lyaste
      Lane 3 : Jurkat cell lyaste
      Lane 4 : K562 cell lyaste

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    • ab109518, at a 1/100 dilution, staining TFIIB in paraffin embedded Human breast tissue by Immunohistochemistry.

    References

    ab109518 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109518.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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