Overview

  • Product name

    Anti-TFIIE alpha/GTF2E1 antibody
    See all TFIIE alpha/GTF2E1 primary antibodies
  • Description

    Rabbit polyclonal to TFIIE alpha/GTF2E1
  • Host species

    Rabbit
  • Specificity

    ab28177 recognises TFIIE alpha/GTF2E1.

  • Tested applications

    Suitable for: WB, ChIPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human TFIIE alpha/GTF2E1.

  • Positive control

    • HeLa nuclear extract
  • General notes

     This product was previously labelled as TFIIE alpha

     

Properties

Applications

Our Abpromise guarantee covers the use of ab28177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
ChIP Use 5-10 µg for 25 µg of chromatin.

Target

  • Function

    Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • Sequence similarities

    Belongs to the TFIIE alpha subunit family.
    Contains 1 HTH TFE/IIEalpha-type domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FE antibody
    • General transcription factor IIE 56 kDa subunit antibody
    • General transcription factor IIE subunit 1 antibody
    • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
    • Gtf2e1 antibody
    • MAL7P1.86 antibody
    • T2EA_HUMAN antibody
    • TF2E1 antibody
    • TFIIE A antibody
    • TFIIE alpha antibody
    • TFIIE-alpha antibody
    • Transcription initiation factor IIE alpha subunit antibody
    • Transcription initiation factor iie alpha subunit putative antibody
    • Transcription initiation factor IIE subunit alpha antibody
    see all

Images

  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

References

ab28177 has not yet been referenced specifically in any publications.

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