RecombinantRabMAb

Recombinant Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)

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Western blot - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
  • Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free
    See all TFIIE alpha/GTF2E1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248917 is the carrier-free version of ab140634 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248917 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TFIIE alpha

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

    • Sequence similarities

      Belongs to the TFIIE alpha subunit family.
      Contains 1 HTH TFE/IIEalpha-type domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • FE antibody
      • General transcription factor IIE 56 kDa subunit antibody
      • General transcription factor IIE subunit 1 antibody
      • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
      • Gtf2e1 antibody
      • MAL7P1.86 antibody
      • T2EA_HUMAN antibody
      • TF2E1 antibody
      • TFIIE A antibody
      • TFIIE alpha antibody
      • TFIIE-alpha antibody
      • Transcription initiation factor IIE alpha subunit antibody
      • Transcription initiation factor iie alpha subunit putative antibody
      • Transcription initiation factor IIE subunit alpha antibody
      see all

    Images

    • Western blot - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      Western blot - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      All lanes : Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634) at 1/1000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 49 kDa



      This data was developed using ab140634, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TFIIE alpha/GTF2E1 with ab140634 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Normal Human Testis tissue using ab140634 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140634 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab140634 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140634 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
      Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248917? Please let us know so that we can cite the reference in this datasheet.

    ab248917 has not yet been referenced specifically in any publications.

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