Recombinant Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free
See all TFIIE alpha/GTF2E1 primary antibodies -
Description
Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248917 is the carrier-free version of ab140634 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248917 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as TFIIE alpha
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8377(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
Target
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Function
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. -
Sequence similarities
Belongs to the TFIIE alpha subunit family.
Contains 1 HTH TFE/IIEalpha-type domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2960 Human
- Entrez Gene: 74197 Mouse
- Entrez Gene: 303918 Rat
- Omim: 189962 Human
- SwissProt: P29083 Human
- SwissProt: Q9D0D5 Mouse
- Unigene: 445272 Human
- Unigene: 287795 Mouse
see all -
Alternative names
- FE antibody
- General transcription factor IIE 56 kDa subunit antibody
- General transcription factor IIE subunit 1 antibody
see all
Images
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All lanes : Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] (ab140634) at 1/1000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 49 kDaThis data was developed using ab140634, the same antibody clone in a different buffer formulation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TFIIE alpha/GTF2E1 with ab140634 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Normal Human Testis tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)This data was developed using ab140634, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140634 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab248917 has not yet been referenced specifically in any publications.