Recombinant Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free (ab248917)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TFIIE alpha/GTF2E1 antibody [EPR8377(2)] - BSA and Azide free
See all TFIIE alpha/GTF2E1 primary antibodies -
Description
Rabbit monoclonal [EPR8377(2)] to TFIIE alpha/GTF2E1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TFIIE alpha/GTF2E1 aa 400-500. The exact sequence is proprietary.
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General notes
ab248917 is the carrier-free version of ab140634 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248917 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as TFIIE alpha
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8377(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab248917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
Target
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Function
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. -
Sequence similarities
Belongs to the TFIIE alpha subunit family.
Contains 1 HTH TFE/IIEalpha-type domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2960 Human
- Entrez Gene: 74197 Mouse
- Entrez Gene: 303918 Rat
- Omim: 189962 Human
- SwissProt: P29083 Human
- SwissProt: Q9D0D5 Mouse
- Unigene: 445272 Human
- Unigene: 287795 Mouse
see all -
Alternative names
- FE antibody
- General transcription factor IIE 56 kDa subunit antibody
- General transcription factor IIE subunit 1 antibody
see all
Datasheets and documents
References
ab248917 has not yet been referenced specifically in any publications.
