Anti-TFIIF antibody (ab15862)
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- References
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Overview
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Product nameAnti-TFIIF antibody
See all TFIIF primary antibodies -
DescriptionChicken polyclonal to TFIIF
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Host speciesChicken
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to amino acids 1-249 of Human TFIIF.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferConstituent: PBS
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Concentration information loading...
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PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgY
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Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab15862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/2000. Predicted molecular weight: 75 kDa. |
Target
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RelevanceThe RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 2962 Human
- Entrez Gene: 2963 Human
- Entrez Gene: 68705 Mouse
- Entrez Gene: 98053 Mouse
- Entrez Gene: 316123 Rat
- Entrez Gene: 81674 Rat
- Omim: 189968 Human
- Omim: 189969 Human
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Alternative names
- ATP dependent helicase GTF2F2 antibody
- BTF 4 antibody
- BTF4 antibody
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Images
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E coli derived fusion protein as test antigen. Affinity purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
E coli derived fusion protein as test antigen. Affinity purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
Protocols
Datasheets and documents
References
ab15862 has not yet been referenced specifically in any publications.