Key features and details
- Rabbit polyclonal to TFIIF
- Suitable for: WB, ChIP
- Isotype: IgG
Product nameAnti-TFIIF antibody
See all TFIIF primary antibodies
DescriptionRabbit polyclonal to TFIIF
SpecificityThis antibody reacts with the RAP74 subunit of TFIIF.
Tested applicationsSuitable for: WB, ChIPmore details
Recombinant full length protein - RAP 74 subunit of TF11F.
- HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab28179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 58 kDa.|
|ChIP||Use 5-10 µg for 25 µg of chromatin.|
RelevanceThe RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
- ATP dependent helicase GTF2F2 antibody
- BTF 4 antibody
- BTF4 antibody
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab28179 has been referenced in 2 publications.
- Hogel M et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). PubMed: 22815947
- Diamant G et al. DSIF restricts NF-?B signaling by coordinating elongation with mRNA processing of negative feedback genes. Cell Rep 2:722-31 (2012). WB, ChIP ; Human . PubMed: 23041311