Anti-TFIIF antibody (ab28180)
Key features and details
- Rabbit polyclonal to TFIIF
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFIIF antibody
See all TFIIF primary antibodies -
Description
Rabbit polyclonal to TFIIF -
Host species
Rabbit -
Specificity
This antibody is specific to the RAP30 subunit of TFIIF. -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
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Immunogen
Recombinant full length protein - RAP30 subunit of the TFIIF complex.
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Positive control
- HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.9
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ChIP |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| ChIP |
Use 5-10 µg for 25 µg of chromatin.
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| Notes |
|---|
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ChIP
Use 5-10 µg for 25 µg of chromatin. |
Target
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Relevance
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2962 Human
- Entrez Gene: 2963 Human
- Omim: 189968 Human
- Omim: 189969 Human
- SwissProt: P13984 Human
- SwissProt: P35269 Human
- Unigene: 654582 Human
- Unigene: 68257 Human
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Alternative names
- ATP dependent helicase GTF2F2 antibody
- BTF 4 antibody
- BTF4 antibody
see all
Images
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ChIP - Anti-TFIIF antibody (ab28180)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28180 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab28180 has been referenced in 2 publications.
- Kalkat M et al. MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis. Mol Cell 72:836-848.e7 (2018). PubMed: 30415952
- Hogel M et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). PubMed: 22815947