Anti-TFIIF antibody (ab28180)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-TFIIF antibody
See all TFIIF primary antibodies -
DescriptionRabbit polyclonal to TFIIF
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Host speciesRabbit
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SpecificityThis antibody is specific to the RAP30 subunit of TFIIF.
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Tested applicationsSuitable for: WB, ChIPmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein - RAP30 subunit of the TFIIF complex.
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Positive control
- HeLa cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab28180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/5000. Predicted molecular weight: 84 kDa. | |
| ChIP | Use 5-10 µg for 25 µg of chromatin. |
Target
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RelevanceThe RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 2962 Human
- Entrez Gene: 2963 Human
- Omim: 189968 Human
- Omim: 189969 Human
- SwissProt: P13984 Human
- SwissProt: P35269 Human
- Unigene: 654582 Human
- Unigene: 68257 Human
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Alternative names
- ATP dependent helicase GTF2F2 antibody
- BTF 4 antibody
- BTF4 antibody
see all
Images
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ChIP - Anti-TFIIF antibody (ab28180)Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28180 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
Datasheets and documents
References
This product has been referenced in:
- Hogel M et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). Read more (PubMed: 22815947) »
