Key features and details
- Rabbit polyclonal to TFIIF
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TFIIF antibody
See all TFIIF primary antibodies
DescriptionRabbit polyclonal to TFIIF
SpecificityThis antibody is specific to the RAP30 subunit of TFIIF.
Tested Applications & Species
Application Species ChIPHuman
Recombinant full length protein - RAP30 subunit of the TFIIF complex.
- HeLa cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.9
Concentration information loading...
PurityProtein A purified
ChIP Related Products
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use 5-10 µg for 25 µg of chromatin.
Use 5-10 µg for 25 µg of chromatin.
RelevanceThe RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
- ATP dependent helicase GTF2F2 antibody
- BTF 4 antibody
- BTF4 antibody
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28180 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab28180 has been referenced in 2 publications.
- Kalkat M et al. MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis. Mol Cell 72:836-848.e7 (2018). PubMed: 30415952
- Hogel M et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). PubMed: 22815947