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    tfiif-antibody-ab28180.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription
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Anti-TFIIF antibody (ab28180)

  • Datasheet
  • SDS
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ChIP - Anti-TFIIF antibody (ab28180)

    Key features and details

    • Rabbit polyclonal to TFIIF
    • Suitable for: ChIP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant Human TFIIF protein (ab81891)

    View more associated products

    Overview

    • Product name

      Anti-TFIIF antibody
      See all TFIIF primary antibodies
    • Description

      Rabbit polyclonal to TFIIF
    • Host species

      Rabbit
    • Specificity

      This antibody is specific to the RAP30 subunit of TFIIF.
    • Tested Applications & Species

      Application Species
      ChIP
      Human
      See all applications and species data
    • Immunogen

      Recombinant full length protein - RAP30 subunit of the TFIIF complex.

    • Positive control

      • HeLa cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.9
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Polymerase associated factors
      • Pol II Transcription
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Polymerase associated factors
      • Pol II Transcription
      • TFIIF

    Associated products

    • ChIP Related Products

      • Rabbit Anti-Mouse IgG H&L (ab46540)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human TFIIF protein (ab81891)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab28180 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ChIP
    Human
    Application Abreviews Notes
    ChIP
    Use 5-10 µg for 25 µg of chromatin.
    Notes
    ChIP
    Use 5-10 µg for 25 µg of chromatin.

    Target

    • Relevance

      The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
    • Cellular localization

      Nuclear
    • Database links

      • Entrez Gene: 2962 Human
      • Entrez Gene: 2963 Human
      • Omim: 189968 Human
      • Omim: 189969 Human
      • SwissProt: P13984 Human
      • SwissProt: P35269 Human
      • Unigene: 654582 Human
      • Unigene: 68257 Human
      • Alternative names

        • ATP dependent helicase GTF2F2 antibody
        • BTF 4 antibody
        • BTF4 antibody
        • General transcription factor IIF polypeptide 1 antibody
        • General transcription factor IIF polypeptide 1 74 kDa subunit protein antibody
        • General transcription factor IIF polypeptide 1 74kDa antibody
        • General transcription factor IIF polypeptide 2 30kDa antibody
        • General Transcription Factor IIF Polypeptide 2 antibody
        • GTF2F1 antibody
        • GTF2F1 protein antibody
        • GTF2F2 antibody
        • RAP 30 antibody
        • RAP 74 antibody
        • RAP30 antibody
        • RAP74 antibody
        • TF2F 1 antibody
        • TF2F 2 antibody
        • TF2F1 antibody
        • TF2F2 antibody
        • TFIIF alpha antibody
        • TFIIF beta antibody
        • Transcription initiation factor IIF alpha subunit antibody
        • Transcription Initiation Factor IIF beta Subunit antibody
        • Transcription initiation factor IIF subunit beta antibody
        • Transcription initiation factor RAP30 antibody
        • Transcription initiation factor RAP74 antibody
        see all

      Images

      • ChIP - Anti-TFIIF antibody (ab28180)
        ChIP - Anti-TFIIF antibody (ab28180)

        Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28180 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.

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      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab28180? Please let us know so that we can cite the reference in this datasheet.

      ab28180 has been referenced in 2 publications.

      • Kalkat M  et al. MYC Protein Interactome Profiling Reveals Functionally Distinct Regions that Cooperate to Drive Tumorigenesis. Mol Cell 72:836-848.e7 (2018). PubMed: 30415952
      • Hogel M  et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). PubMed: 22815947

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