Key features and details
- Rabbit polyclonal to TFIIIC / GTF3C1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TFIIIC / GTF3C1 antibody
DescriptionRabbit polyclonal to TFIIIC / GTF3C1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 2059 and 2109 of human TFIIIC/ GTF3C1 protein (NP_001511.2)
- HeLa and 293T whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75251 was affinity purified using an epitope specific to TFIIIC / GTF3C1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab75251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 238 kDa.|
RelevanceThe transcription factor IIIC (TFIIIC) is a multi-subunit DNA-binding factor required for promoter recognition and TFIIIB assembly on tRNA genes transcribed by RNA polymerase III.
- General transcription factor 3C polypeptide 1 antibody
- GTF3C1 antibody
- TF3C-alpha antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TFIIIC / GTF3C1 with ab75251 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-TFIIIC / GTF3C1 antibody (ab75251) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 238 kDa
Observed band size: 238 kDa
Exposure time: 3 minutes
ab75251 has not yet been referenced specifically in any publications.