Overview

  • Product name

  • Description

    Rabbit polyclonal to TFIISH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to Human TFIISH aa 1-73. Numbering is from isoform 1; the sequence also occurs in isoform 2.
    Sequence:

    MGQEEELLRIAKKLEKMVARKNTEGALDLLKKLHSCQMSIQLLQTTRIGV AVNGVRKHCSDKEVVSLAKVLIK


    Database link: O75764

  • Positive control

    • U-2 OS cells.
  • General notes

    Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Properties

Applications

Our Abpromise guarantee covers the use of ab220771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Sequence similarities

    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Rhabdomyosarcoma antigen MU RMS 40.22 antibody
    • Tcea3 antibody
    • TCEA3_HUMAN antibody
    • TFIIS antibody
    • TFIIS.H antibody
    • Transcription elongation factor A (SII) 3 antibody
    • Transcription elongation factor A protein 3 antibody
    • Transcription elongation factor A3 antibody
    • Transcription elongation factor S-II protein 3 antibody
    • Transcription elongation factor TFIIS.h antibody
    see all

Images

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling TFIISH with ab220771 at 4 µg/ml.  Antibody staining is shown in green.  

References

ab220771 has not yet been referenced specifically in any publications.

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