Product nameAnti-TFIISH antibody [TCE5I551]
See all TFIISH primary antibodies
DescriptionMouse monoclonal [TCE5I551] to TFIISH
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Recombinant human TFIISH
- WB: Hela nuclear extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityProtein G purified
Purification notesab50797 from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab50797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).|
|ELISA||Use at an assay dependent dilution.|
FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Sequence similaritiesBelongs to the TFS-II family.
Contains 1 TFIIS central domain.
Contains 1 TFIIS N-terminal domain.
Contains 1 TFIIS-type zinc finger.
- Information by UniProt
- Rhabdomyosarcoma antigen MU RMS 40.22 antibody
- Tcea3 antibody
- TCEA3_HUMAN antibody
Anti-TFIISH antibody [TCE5I551] (ab50797) at 1/100 dilution + HeLa nuclear extract at 20 µg/lane
Mouse IgG antibody at 1/3000 dilution
Predicted band size: 39 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
ab50797 has not yet been referenced specifically in any publications.