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Anti-TFPI antibody (ab180619)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI antibody (ab180619)
  • Western blot - Anti-TFPI antibody (ab180619)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI antibody (ab180619)
  • Immunocytochemistry/ Immunofluorescence - Anti-TFPI antibody (ab180619)
  • Western blot - Anti-TFPI antibody (ab180619)

Key features and details

  • Rabbit polyclonal to TFPI
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TFPI antibody
    See all TFPI primary antibodies
  • Description

    Rabbit polyclonal to TFPI
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human TFPI aa 29-224.
    Sequence:

    DSEEDEEHTIITDTELPPLKLMHSFCAFKADDGPCKAIMKRFFFNIFTRQ CEEFIYGGCEGNQNRFESLEECKKMCTRDNANRIIKTTLQQEKPDFCFLE EDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDG PNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRG


    Database link: P10646
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HepG2 and HeLa cell line extracts.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Extrinsic

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human TFPI protein (ab167711)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 35 kDa.
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 35 kDa.
IHC-P
1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • Tissue specificity

    Mostly in endothelial cells.
  • Sequence similarities

    Contains 3 BPTI/Kunitz inhibitor domains.
  • Domain

    This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P10646 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7035 Human
    • Entrez Gene: 21788 Mouse
    • Omim: 152310 Human
    • SwissProt: P10646 Human
    • SwissProt: O54819 Mouse
    • Unigene: 516578 Human
    • Unigene: 124316 Mouse
    • Unigene: 447013 Mouse
    • Alternative names

      • Anti convertin antibody
      • EPI antibody
      • Extrinsic pathway inhibitor antibody
      • LACI antibody
      • Lipoprotein associated coagulation inhibitor antibody
      • Lipoprotein-associated coagulation inhibitor antibody
      • TFI antibody
      • TFPI 1 antibody
      • TFPI antibody
      • TFPI1 antibody
      • TFPI1_HUMAN antibody
      • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) antibody
      • Tissue factor pathway inhibitor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI antibody (ab180619)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI antibody (ab180619)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling TFPI with ab180619 at 1/100. Magnification: 200x.
    • Western blot - Anti-TFPI antibody (ab180619)
      Western blot - Anti-TFPI antibody (ab180619)
      All lanes : Anti-TFPI antibody (ab180619) at 1/500 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : Mouse lung tissue lysate
      Lane 4 : Mouse heart tissue lysate
      Lane 5 : Mouse spleen tissue lysate

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI antibody (ab180619)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI antibody (ab180619)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling TFPI with ab180619 at 1/100. Magnification: 200x.
    • Immunocytochemistry/ Immunofluorescence - Anti-TFPI antibody (ab180619)
      Immunocytochemistry/ Immunofluorescence - Anti-TFPI antibody (ab180619)
      Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180619. Blue DAPI for nuclear staining.
    • Western blot - Anti-TFPI antibody (ab180619)
      Western blot - Anti-TFPI antibody (ab180619)
      All lanes : Anti-TFPI antibody (ab180619) at 1/500 dilution

      Lane 1 : HepG2 cell line extract
      Lane 2 : HeLa cell line extract

      Predicted band size: 35 kDa

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab180619? Please let us know so that we can cite the reference in this datasheet.

    ab180619 has been referenced in 3 publications.

    • Ma J  et al. Role of the TGFß/PDCD4/AP-1 Signaling Pathway in Nasopharyngeal Carcinoma and Its Relationship to Prognosis. Cell Physiol Biochem 43:1392-1401 (2017). PubMed: 29017171
    • Chen X  et al. Comparative Profiling of Triple-Negative Breast Carcinomas Tissue Glycoproteome by Sequential Purification of Glycoproteins and Stable Isotope Labeling. Cell Physiol Biochem 38:110-21 (2016). PubMed: 26742121
    • Abu El-Asrar AM  et al. Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy. Mol Vis 22:424-35 (2016). WB, IHC . PubMed: 27168718

    Customer reviews and Q&As

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