Anti-TFPI antibody (ab66544)
Key features and details
- Rabbit polyclonal to TFPI
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFPI antibody
See all TFPI primary antibodies -
Description
Rabbit polyclonal to TFPI -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide corresponding to Human TFPI aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab66543) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 35 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 35 kDa). |
Target
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7035 Human
- Entrez Gene: 29436 Rat
- Entrez Gene: 613026 Rhesus monkey
- Omim: 152310 Human
- SwissProt: P10646 Human
- SwissProt: Q02445 Rat
- SwissProt: Q28864 Rhesus monkey
- Unigene: 516578 Human
see all -
Alternative names
- Anti convertin antibody
- EPI antibody
- Extrinsic pathway inhibitor antibody
see all
Images
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All lanes : Anti-TFPI antibody (ab66544) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
This protein contains a number of potential glycosylation sites (SwissProt). The 52 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to TFPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab66544 has been referenced in 2 publications.
- Cheng X et al. Trimethylamine N-oxide promotes tissue factor expression and activity in vascular endothelial cells: A new link between trimethylamine N-oxide and atherosclerotic thrombosis. Thromb Res 177:110-116 (2019). PubMed: 30875490
- Liczko J et al. Tissue factor and tissue factor pathway inhibitor in chronically inflamed gallbladder mucosa. Biomed Res Int 2014:403639 (2014). IHC-Fr ; Human . PubMed: 24716194