Key features and details
- Mouse monoclonal [C1] to TFPI
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2a
Product nameAnti-TFPI antibody [C1]
See all TFPI primary antibodies
DescriptionMouse monoclonal [C1] to TFPI
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Recombinant full length protein corresponding to Rat TFPI aa 29-302. N-terminal His tag. Expressed in E. coli. Full length mature chain without the signal peptide.
LPEEDDDTINTDSELRPMKPLHTFCAMKAEDGPCKAMIRSYYFNMNSHQC EEFIYGGCRGNKNRFDTLEECRKTCIPGYKKTTIKTTSGAEKPDFCFLEE DPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPV NEVQKGDYVTNQITVTDRTTVNNVVIPQATKAPSQWDYDGPSWCLEPADS GLCKASEKRFYYNPAIGKCRQFNYTGCGGNNNNFTTKQDCNRACKKDSSK KSSKRAKTQRRRKSFVKVMYENIH
Database link: Q02445
- WB: Recombinant rat TFPI protein. HeLa and HepG2 cell lysate. IHC-P: Rat skin and stomach tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified by TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab239516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml.|
|IHC-P||Use a concentration of 5 - 30 µg/ml.|
FunctionInhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
Tissue specificityMostly in endothelial cells.
Sequence similaritiesContains 3 BPTI/Kunitz inhibitor domains.
DomainThis inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
- Information by UniProt
- Anti convertin antibody
- EPI antibody
- Extrinsic pathway inhibitor antibody
Formalin-fixed, paraffin-embedded rat skin tissue stained for TFPI with ab239516 at 20 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Anti-TFPI antibody [C1] (ab239516) at 5 µg/ml + Recombinant rat TFPI protein
Formalin-fixed, paraffin-embedded rat stomach tissue stained for TFPI with ab239516 at 20 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
Anti-TFPI antibody [C1] (ab239516) at 3 µg/ml + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239516 has not yet been referenced specifically in any publications.