Recombinant Anti-TFPI antibody [EPR7941] (ab134151)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7941] to TFPI
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TFPI antibody [EPR7941]
See all TFPI primary antibodies -
Description
Rabbit monoclonal [EPR7941] to TFPI -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TFPI aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HepG2, U87-MG and Caco-2 cell lysates and human placenta tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7941 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
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Alternative names
- Anti convertin antibody
- EPI antibody
- Extrinsic pathway inhibitor antibody
see all
Images
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All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution (purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : Human placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab134151 has been referenced in 1 publication.
- Papareddy P et al. An ecoimmunological approach to study evolutionary and ancient links between coagulation, complement and Innate immunity. Virulence 9:724-737 (2018). PubMed: 29473457