Recombinant Anti-TFPI antibody [EPR7941] (ab134151)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TFPI antibody [EPR7941]
See all TFPI primary antibodies -
Description
Rabbit monoclonal [EPR7941] to TFPI -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TFPI aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HepG2, U87-MG and Caco-2 cell lysates and human placenta tissue lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7941 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
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Alternative names
- Anti convertin antibody
- EPI antibody
- Extrinsic pathway inhibitor antibody
see all
Images
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![Western blot - Anti-TFPI antibody [EPR7941] (ab134151)](https://www.abcam.com/ps/products/134/ab134151/Images/ab134151-241155-anti-tfpi-antibody-epr7941-western-blot.jpg)
Western blot - Anti-TFPI antibody [EPR7941] (ab134151)All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution (purified)
Lane 1 : U87-MG cell lysate
Lane 2 : Human placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-TFPI antibody [EPR7941] (ab134151)](https://www.abcam.com/ps/products/134/ab134151/Images/ab134151-241156-anti-tfpi-antibody-epr7941-western-blot.jpg)
Western blot - Anti-TFPI antibody [EPR7941] (ab134151)Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-TFPI antibody [EPR7941] (ab134151)](https://www.abcam.com/ps/products/134/ab134151/Images/ab134151-148420-anti-tfpi-antibody-epr7941-western-blot.jpg)
Western blot - Anti-TFPI antibody [EPR7941] (ab134151)All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Papareddy P et al. An ecoimmunological approach to study evolutionary and ancient links between coagulation, complement and Innate immunity. Virulence 9:724-737 (2018). Read more (PubMed: 29473457) »
