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RecombinantRabMAb

Recombinant Anti-TFPI antibody [EPR7941] (ab134151)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
  • Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
  • Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
  • Anti-TFPI antibody [EPR7941] (ab134151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7941] to TFPI
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant human TFPI protein (ab167711)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TFPI antibody [EPR7941]
    See all TFPI primary antibodies
  • Description

    Rabbit monoclonal [EPR7941] to TFPI
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TFPI aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, U87-MG and Caco-2 cell lysates and human placenta tissue lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7941
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Extrinsic

Associated products

  • Alternative Versions

    • Anti-TFPI antibody [EPR7941] - BSA and Azide free (ab248703)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Caco-2 whole cell lysate (ab3950)
  • Recombinant Protein

    • Recombinant human TFPI protein (ab167711)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
    • Tissue specificity

      Mostly in endothelial cells.
    • Sequence similarities

      Contains 3 BPTI/Kunitz inhibitor domains.
    • Domain

      This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
    • Post-translational
      modifications

      O-glycosylated.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P10646 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7035 Human
      • Omim: 152310 Human
      • SwissProt: P10646 Human
      • Unigene: 516578 Human
      • Alternative names

        • Anti convertin antibody
        • EPI antibody
        • Extrinsic pathway inhibitor antibody
        • LACI antibody
        • Lipoprotein associated coagulation inhibitor antibody
        • Lipoprotein-associated coagulation inhibitor antibody
        • TFI antibody
        • TFPI 1 antibody
        • TFPI antibody
        • TFPI1 antibody
        • TFPI1_HUMAN antibody
        • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) antibody
        • Tissue factor pathway inhibitor antibody
        see all

      Images

      • Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
        Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
        All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution (purified)

        Lane 1 : U87-MG cell lysate
        Lane 2 : Human placenta tissue lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

        Predicted band size: 35 kDa
        Observed band size: 40-45 kDa
        why is the actual band size different from the predicted?



        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
        Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
        Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution (purified) + HepG2 cell lysate at 20 µg

        Secondary
        HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

        Predicted band size: 35 kDa
        Observed band size: 40-45 kDa why is the actual band size different from the predicted?



        Blocking buffer and concentration: 5% NFDM/TBST.

        Diluting buffer and concentration: 5% NFDM /TBST.

      • Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
        Western blot - Anti-TFPI antibody [EPR7941] (ab134151)
        All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution (unpurified)

        Lane 1 : HepG2 cell lysate
        Lane 2 : U87-MG cell lysate
        Lane 3 : Caco-2 cell lysate
        Lane 4 : Human placenta tissue lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 35 kDa

      • Anti-TFPI antibody [EPR7941] (ab134151)
        Anti-TFPI antibody [EPR7941] (ab134151)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab134151? Please let us know so that we can cite the reference in this datasheet.

      ab134151 has been referenced in 1 publication.

      • Papareddy P  et al. An ecoimmunological approach to study evolutionary and ancient links between coagulation, complement and Innate immunity. Virulence 9:724-737 (2018). PubMed: 29473457

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab134151.
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