Overview

  • Product name
    Anti-TFPI antibody [EPR7941]
    See all TFPI primary antibodies
  • Description
    Rabbit monoclonal [EPR7941] to TFPI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TFPI (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: HepG2, U87-MG and Caco-2 cell lysates and human placenta tissue lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR7941
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
    • Tissue specificity
      Mostly in endothelial cells.
    • Sequence similarities
      Contains 3 BPTI/Kunitz inhibitor domains.
    • Domain
      This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anti convertin antibody
      • EPI antibody
      • Extrinsic pathway inhibitor antibody
      • LACI antibody
      • Lipoprotein associated coagulation inhibitor antibody
      • Lipoprotein-associated coagulation inhibitor antibody
      • TFI antibody
      • TFPI 1 antibody
      • TFPI antibody
      • TFPI1 antibody
      • TFPI1_HUMAN antibody
      • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) antibody
      • Tissue factor pathway inhibitor antibody
      see all

    Images

    • All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution (purified)

      Lane 1 : U87-MG cell lysate
      Lane 2 : Human placenta tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 35 kDa
      Observed band size: 40-45 kDa
      why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution (purified) + HepG2 cell lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 35 kDa
      Observed band size: 40-45 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : Caco-2 cell lysate
      Lane 4 : Human placenta tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 35 kDa

    References

    This product has been referenced in:
    • Papareddy P  et al. An ecoimmunological approach to study evolutionary and ancient links between coagulation, complement and Innate immunity. Virulence 9:724-737 (2018). Read more (PubMed: 29473457) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134151.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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