Anti-TFPI antibody [EPR7941] (ab134151)
- Datasheet
- References (1)
- Protocols
Overview
-
Product nameAnti-TFPI antibody [EPR7941]
See all TFPI primary antibodies -
DescriptionRabbit monoclonal [EPR7941] to TFPI
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human TFPI (C terminal). The exact sequence is proprietary.
-
Positive control
- WB: HepG2, U87-MG and Caco-2 cell lysates and human placenta tissue lysate.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEPR7941
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab134151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
-
FunctionInhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
-
Tissue specificityMostly in endothelial cells.
-
Sequence similaritiesContains 3 BPTI/Kunitz inhibitor domains.
-
DomainThis inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
-
Post-translational
modificationsO-glycosylated. -
Cellular localizationSecreted.
- Information by UniProt
-
Database links
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
-
Alternative names
- Anti convertin antibody
- EPI antibody
- Extrinsic pathway inhibitor antibody
see all
Images
-
![Western blot - Anti-TFPI antibody [EPR7941] (ab134151)](https://www.abcam.com/ps/products/134/ab134151/Images/ab134151-241155-ab134151wb.jpg)
Western blot - Anti-TFPI antibody [EPR7941] (ab134151)All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution (purified)
Lane 1 : U87-MG cell lysate
Lane 2 : Human placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-TFPI antibody [EPR7941] (ab134151)](https://www.abcam.com/ps/products/134/ab134151/Images/ab134151-241156-ab134151wb2.jpg)
Western blot - Anti-TFPI antibody [EPR7941] (ab134151)Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-TFPI antibody [EPR7941] (ab134151)](https://www.abcam.com/ps/datasheet/images/134/ab134151/TFPI-Primary-antibodies-ab134151-2.jpg)
Western blot - Anti-TFPI antibody [EPR7941] (ab134151)All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Papareddy P et al. An ecoimmunological approach to study evolutionary and ancient links between coagulation, complement and Innate immunity. Virulence 9:724-737 (2018). Read more (PubMed: 29473457) »
