Recombinant Anti-TFPI antibody [EPR7941] - BSA and Azide free (ab248703)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7941] to TFPI - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TFPI antibody [EPR7941] - BSA and Azide free
See all TFPI primary antibodies -
Description
Rabbit monoclonal [EPR7941] to TFPI - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TFPI (C terminal). The exact sequence is proprietary.
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General notes
ab248703 is the carrier-free version of ab134151.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7941 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
Target
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
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Alternative names
- Anti convertin antibody
- EPI antibody
- Extrinsic pathway inhibitor antibody
see all
Images
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All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/2000 dilution (purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : Human placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?This data was developed using ab134151, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-TFPI antibody [EPR7941] (ab134151) at 1/10000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?This data was developed using ab134151, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-TFPI antibody [EPR7941] (ab134151) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDaThis data was developed using ab134151, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248703 has not yet been referenced specifically in any publications.