Overview

  • Product name
    Anti-TFPI2 antibody [EPR14442]
    See all TFPI2 primary antibodies
  • Description
    Rabbit monoclonal [EPR14442] to TFPI2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TFPI2 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P48307

  • Positive control
    • Human kidney tissue sections. Jurkat, MCF7, rat spleen, HeLa, mouse heart, mouse brain, mouse kidney and mouse spleen lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40 - 1/60.
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
  • Tissue specificity
    Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.
  • Sequence similarities
    Contains 3 BPTI/Kunitz inhibitor domains.
  • Domain
    This inhibitor contains three inhibitory domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Placental protein 5 antibody
    • PP5 antibody
    • REF1 antibody
    • Retinal pigment epithelium cell factor 1 antibody
    • TFPI 2 antibody
    • TFPI-2 antibody
    • TFPI2 antibody
    • TFPI2 PEN antibody
    • TFPI2_HUMAN antibody
    • Tissue factor pathway inhibitor 2 antibody
    see all

Images

  • All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/10000 dilution

    Lane 1 : rat spleen lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling TFPI2 using ab186747 at a 1/250 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Lysate from HeLa cells was immunoprecipitated with ab186747 at a 1/50 dilution. For the subsequent blot, ab186747 was used at a 1/1000 dilution, with a 1/1000 dilution of Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated as the secondary antibody. Blocking/ Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:
  • Tsuchiya H & Oura S Involvement of MAFB and MAFF in Retinoid-Mediated Suppression of Hepatocellular Carcinoma Invasion. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29757260) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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