Overview

  • Product name

    Anti-TG6 antibody [EPR10308] - N-terminal
    See all TG6 primary antibodies
  • Description

    Rabbit monoclonal [EPR10308] to TG6 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TG6 aa 1-100. The exact sequence is proprietary.
    Database link: O95932

  • Positive control

    • MOLT4 cell lysate; Human fetal kidney lysate; Human adenocarcinoma of endometrium tissue; MOLT4 cells
  • General notes

     

     

    Previously labelled as Transglutaminase 6. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • Involvement in disease

    Defects in TGM6 are the cause of spinocerebellar ataxia type 35 (SCA35) [MIM:613908]. A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA35 patients commonly show upper limb involvement and torticollis. There is no cognitive impairment.
  • Sequence similarities

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein-glutamine gamma-glutamyltransferase 6 antibody
    • TG6 antibody
    • TGase Y antibody
    • TGase-3-like antibody
    • TGase-6 antibody
    • TGM3L_HUMAN antibody
    • TGM6 antibody
    • TGY antibody
    • Transglutaminase 6 antibody
    • Transglutaminase Y antibody
    • Transglutaminase-3-like antibody
    • Transglutaminase-6 antibody
    see all

Images

  • Flow cytometric analysis of 2% paraformaldehyde fixed MOLT4 cells labelling TG6 (green) or negative control (red) using ab180959 at 1/20 dlution followed by Goat anti rabbit IgG (FITC) at 1/150 diltuion.

  • Immunohistochemical analysis of paraffin- embedded Human adenocarcinoma of endometrium tissue labeling TG6 with ab180959 at 1/100 dilution. Secondary antibody ImmunoHistoprobe HRP Polymer for Rabbit IgG pre-diluted.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/5000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa

  • Anti-TG6 antibody [EPR10308] - N-terminal (ab180959) at 1/2000 dilution + MOLT4 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa

References

ab180959 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180959.
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