• Product name
  • Description
    Rabbit polyclonal to TGE
  • Host species
  • Specificity
    This antibody is specific to TGE from epidermal cell.
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TGE.

  • Positive control
    • Human epidermal extract
  • General notes

    Protein previously labeled as Transglutaminase 3.



Our Abpromise guarantee covers the use of ab27001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21335491
WB 1/200 - 1/1000. Predicted molecular weight: 76 kDa.


  • Function
    Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
  • Sequence similarities
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075).
  • Information by UniProt
  • Database links
  • Alternative names
    • E polypeptide protein glutamine gamma glutamyltransferase antibody
    • MGC126249 antibody
    • MGC126250 antibody
    • Protein glutamine gamma glutamyltransferase E 27 kDa catalytic chain antibody
    • Protein glutamine gamma glutamyltransferase E 50 kDa noncatalytic chain antibody
    • Protein glutamine gamma glutamyltransferase E antibody
    • Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain antibody
    • TG(E) antibody
    • TGase E antibody
    • TGase-3 antibody
    • TGE antibody
    • TGM 3 antibody
    • TGM3 antibody
    • TGM3_HUMAN antibody
    • Transglutaminase E antibody
    • Transglutaminase-3 antibody
    • Transglutaminase3 antibody
    • TransglutaminaseE antibody
    see all


  • 20 µg of human epidermal lysate was loaded. Following protein transfer ab27001 was applied at a dilution of 1/500. An anti-Rabbit secondary antibody (non-Abcam) was used at 1/2000. Molecular weight 76 kDa.


This product has been referenced in:
  • Zone JJ  et al. Dermatitis herpetiformis sera or goat anti-transglutaminase-3 transferred to human skin-grafted mice mimics dermatitis herpetiformis immunopathology. J Immunol 186:4474-80 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21335491) »

See 1 Publication for this product

Customer reviews and Q&As

The antibody was validated on different systems including keratinocytes. TG3 zymogen (77kDa) in skin is known to be cleaved (proteolysed) into 47 and 30 kDa. Indeed, this antibody recognizes both full length (77 kDa) and proteolysed form of 50kD.

Thank you for your enquiry. I have mailed the western image as an attachment in an e-mail that follows. Unfortunately the contrast on the blot is not as good as I would hope. The positive control used for the western blot was a Human epidermal extr...

Read More


Sign up