Anti-TGE antibody - C-terminal (ab203229)
Key features and details
- Rabbit polyclonal to TGE - C-terminal
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-TGE antibody - C-terminal
See all TGE primary antibodies -
Description
Rabbit polyclonal to TGE - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Human TGE aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q08188 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution. -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
(or 1/50 – 1/200 using a fluorescent secondary antibody). |
Notes |
---|
IHC-P
1/100 - 1/500. (or 1/50 – 1/200 using a fluorescent secondary antibody). |
Target
-
Function
Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Post-translational
modificationsActivated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075). - Information by UniProt
-
Database links
- Entrez Gene: 21818 Mouse
- SwissProt: Q08189 Mouse
- Unigene: 2961 Mouse
-
Alternative names
- E polypeptide protein glutamine gamma glutamyltransferase antibody
- MGC126249 antibody
- MGC126250 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab203229 has been referenced in 2 publications.
- Aepler J et al. The Role of Transglutaminase 2 in the Radioresistance of Melanoma Cells. Cells 11:N/A (2022). PubMed: 35456021
- Hauser S et al. Application of a Fluorescence Anisotropy-Based Assay to Quantify Transglutaminase 2 Activity in Cell Lysates. Int J Mol Sci 23:N/A (2022). PubMed: 35562866