Overview

  • Product name

    Anti-TGE antibody - N-terminal
    See all TGE primary antibodies
  • Description

    Rabbit polyclonal to TGE - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TGE (N terminal). The exact sequence is proprietary. (Sequence: 180 amino acids).
    Database link: Q08188

  • Positive control

    • BT-474, A549 and U87 cell extracts
  • General notes

    Protein previously labeled as Transglutaminase 3.

Properties

Applications

Our Abpromise guarantee covers the use of ab189830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 76 kDa.

Target

  • Function

    Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
  • Sequence similarities

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    modifications

    Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075).
  • Information by UniProt
  • Database links

  • Alternative names

    • E polypeptide protein glutamine gamma glutamyltransferase antibody
    • MGC126249 antibody
    • MGC126250 antibody
    • Protein glutamine gamma glutamyltransferase E 27 kDa catalytic chain antibody
    • Protein glutamine gamma glutamyltransferase E 50 kDa noncatalytic chain antibody
    • Protein glutamine gamma glutamyltransferase E antibody
    • Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain antibody
    • TG(E) antibody
    • TGase E antibody
    • TGase-3 antibody
    • TGE antibody
    • TGM 3 antibody
    • TGM3 antibody
    • TGM3_HUMAN antibody
    • Transglutaminase E antibody
    • Transglutaminase-3 antibody
    • Transglutaminase3 antibody
    • TransglutaminaseE antibody
    see all

Images

  • All lanes : Anti-TGE antibody - N-terminal (ab189830) at 1/500 dilution

    Lane 1 : BT-474 cell extract
    Lane 2 : A549 cell extract
    Lane 3 : UB87 cell extract

    Predicted band size: 76 kDa

References

ab189830 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization
No
Specification
human skin
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Jun 24 2016

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