Key features and details
- Rabbit polyclonal to TGF alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TGF alpha antibody
See all TGF alpha primary antibodies
DescriptionRabbit polyclonal to TGF alpha
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
Synthetic peptide within Human TGF alpha (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P01135
- WB: TGF alpha transfected HEK-293T whole cell lysate. IHC: Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionTGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Tissue specificityIsoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
Sequence similaritiesContains 1 EGF-like domain.
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
- EGF like TGF antibody
- EGF-like TGF antibody
- ETGF antibody
Paraffin-embedded human breast carcinoma tissue stained for TGF alpha using ab227723 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-TGF alpha antibody (ab227723) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : TGF alpha-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227723 has not yet been referenced specifically in any publications.