Anti-TGF alpha antibody [TG86] (ab233974)
Key features and details
- Mouse monoclonal [TG86] to TGF alpha
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TGF alpha antibody [TG86]
See all TGF alpha primary antibodies -
Description
Mouse monoclonal [TG86] to TGF alpha -
Host species
Mouse -
Specificity
ab233974 reacts with TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Rabbit, Zebrafish, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Human TGF alpha aa 1-100.
Database link: P01135 -
Positive control
- IHC-P: Human testicular carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
TG86 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate for 30 minutes at room temperature. |
Notes |
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate for 30 minutes at room temperature. |
Target
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Function
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 7039 Human
- Entrez Gene: 100009482 Rabbit
- Entrez Gene: 613031 Rhesus monkey
- Entrez Gene: 101106508 Sheep
- Entrez Gene: 100034402 Zebrafish
- Omim: 190170 Human
- SwissProt: P01135 Human
- SwissProt: P55244 Rhesus monkey
see all -
Alternative names
- EGF like TGF antibody
- EGF-like TGF antibody
- ETGF antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233974 has not yet been referenced specifically in any publications.