Recombinant
RabMAb

Recombinant Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)

Overview

  • Product name

    Anti-TGF beta 1 + 2 antibody [EPR5443]
    See all TGF beta 1 + 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5443] to TGF beta 1 + 2
  • Host species

    Rabbit
  • Specificity

    ab124894 recognises TGF beta 1 and 2.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human TGF beta 1. (Uniprot: P01137)

  • Positive control

    • A549 lysate, K562 lysate and L929 lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44, 48 kDa.Can be blocked with Human TGF beta 1 + 2 peptide (ab229618).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Relevance

      Function: TGF beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. The inactive form consists of a TGFB1 homodimer non-covalently linked to a latency-associated peptide (LAP) homodimer. The inactive complex can contain a latent TGFB1-binding protein. The active form is a homodimer of mature TGFB1; disulfide-linked. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Similarity: Both belong to the TGF-beta family.
    • Cellular localization

      Secreted
    • Database links

    • Alternative names

      • TGF-beta-1 antibody
      • TGF-beta-2 antibody
      • Transforming growth factor Beta 1 antibody
      • Transforming growth factor Beta 2 antibody

    Images

    • All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution

      Lane 1 : A549 lysate
      Lane 2 : K562 lysate
      Lane 3 : L929 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44, 48 kDa

    • All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution

      Lane 1 : TGF beta 2 recombinant protein
      Lane 2 : TGF beta 1 recombinant protein

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44, 48 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Huang PS  et al. The Long Non-Coding RNA MIR503HG Enhances Proliferation of Human ALK-Negative Anaplastic Large-Cell Lymphoma. Int J Mol Sci 19:N/A (2018). WB . Read more (PubMed: 29758012) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer


    The lab let me know the following regarding their testing in mouse and rat:
    Even though there is 100% sequence homology with the mouse and rat proteins, we still typically test for cross reactivity before recommending them for use with other species. In this case, ab124894 did not produce a band at the predicted MW in WB with mouse and rat samples (brain, heart kidney, lung and spleen as well as C6, Raw264.7, PC-12, NIH/3T3 and L929 cell lines).

    Read More

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