Recombinant Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5443] to TGF beta 1 + 2
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TGF beta 1 + 2 antibody [EPR5443]
See all TGF beta 1 + 2 primary antibodies -
Description
Rabbit monoclonal [EPR5443] to TGF beta 1 + 2 -
Host species
Rabbit -
Specificity
ab124894 recognises TGF beta 1 and 2. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549 lysate, K562 lysate and L929 lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.64 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5443 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 44, 48 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 44, 48 kDa. |
Target
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Relevance
Function: TGF beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. The inactive form consists of a TGFB1 homodimer non-covalently linked to a latency-associated peptide (LAP) homodimer. The inactive complex can contain a latent TGFB1-binding protein. The active form is a homodimer of mature TGFB1; disulfide-linked. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Similarity: Both belong to the TGF-beta family. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 7040 Human
- Entrez Gene: 7042 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 21808 Mouse
- Omim: 190180 Human
- Omim: 190220 Human
- SwissProt: P01137 Human
- SwissProt: P61812 Human
see all -
Alternative names
- TGF-beta-1 antibody
- TGF-beta-2 antibody
- Transforming growth factor Beta 1 antibody
- Transforming growth factor Beta 2 antibody
Images
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All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : K562 lysate
Lane 3 : L929 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44, 48 kDa -
All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution
Lane 1 : TGF beta 2 recombinant protein
Lane 2 : TGF beta 1 recombinant protein
Lysates/proteins at 10 µg per lane.
Predicted band size: 44, 48 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124894 has been referenced in 1 publication.
- Huang PS et al. The Long Non-Coding RNA MIR503HG Enhances Proliferation of Human ALK-Negative Anaplastic Large-Cell Lymphoma. Int J Mol Sci 19:N/A (2018). WB . PubMed: 29758012