Recombinant Anti-TGF beta 1 + 2 antibody [EPR5443] - BSA and Azide free (ab183133)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5443] to TGF beta 1 + 2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TGF beta 1 + 2 antibody [EPR5443] - BSA and Azide free
See all TGF beta 1 + 2 primary antibodies -
Description
Rabbit monoclonal [EPR5443] to TGF beta 1 + 2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognises TGF beta 1 and 2.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab183133 is the carrier-free version of ab124894.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 8.64 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5443 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 44, 48 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 44, 48 kDa. |
Target
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Relevance
Function: TGF beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. The inactive form consists of a TGFB1 homodimer non-covalently linked to a latency-associated peptide (LAP) homodimer. The inactive complex can contain a latent TGFB1-binding protein. The active form is a homodimer of mature TGFB1; disulfide-linked. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Similarity: Both belong to the TGF-beta family. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 7040 Human
- Entrez Gene: 7042 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 21808 Mouse
- Omim: 190180 Human
- Omim: 190220 Human
- SwissProt: P01137 Human
- SwissProt: P61812 Human
see all -
Alternative names
- TGF-beta-1 antibody
- TGF-beta-2 antibody
- Transforming growth factor Beta 1 antibody
- Transforming growth factor Beta 2 antibody
Images
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All lanes : Anti-TGF beta 1 + 2 antibody [EPR5443] (ab124894) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : K562 lysate
Lane 3 : L929 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44, 48 kDaThis data was developed using ab124894, the same antibody clone in a different buffer formulation.
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This data was developed using ab124894, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab183133 has not yet been referenced specifically in any publications.